3,4-dimethoxyphenylacetonitrile

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Similar to guideline study (OECD 402). Non-GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

BASF Test

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

veratrylcyanid

IUPAC Name:

veratrylcyanid

Constituent 2

Reference substance name:

78/834

IUPAC Name:

78/834

Details on test material:

Veratrylcyanid; no data on purity given in the report

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

- Source: Hagemann
- diet: Herilan MRH
- mean weights at application time: 203 g (females) and 258 g (males).

Administration / exposure

Type of coverage:

not specified

Vehicle:

water

Details on dermal exposure:

- way of application: dermal
- area: 42 cm2 on the back
- form of application: suspension
- formulation in: 50% aqua dest.
- observation period: 14 days

Duration of exposure:

24 h

Doses:

1,000 and 2,000 mg/kg bw.

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: not described in the protocol
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

no mortality observed.

Clinical signs:

Irregular respiration, apathy, staggering, spasms. Slight reversible erythema 24 h after the application.

Body weight:

Mean body weights: 258 g (males) and 203 g (females).

Gross pathology:

No abnormalities observed in the organs of any sacrificed animal.

Any other information on results incl. tables

LD50 > 2000 mg/kg bw.

A 50% aqueous formulation of the test substance was applied. After dosing, the 
rats were observed for 14 days. Clinical signs of toxicity included irregular 
respiration, apathy, staggering and spastic gait. Local (skin) effects were 
limited to slight primary skin irritation that was observed after 24 hours. No 
pathological changes were noted.

Applicant's summary and conclusion

Conclusions:

Based on the results of this study, the test substance veratrylcyanid does not need to be classified according to Regulation (EC) No. 1272/2008 and Directive 67/548/EEC.