Poly (dipropyleneglycol) Phenyl phosphite

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

92/69/EEC C.l

Deviations:

yes

Remarks:

Low oxygen levels (34% of saturation value) occured at concentration 100 mg/L after 48 hours exposure for a few hours. No analytical determination of test substance concentrations.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]

EC Number:

279-499-4

EC Name:

Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]

Cas Number:

80584-86-7

Molecular formula:

C102H134O31P8

IUPAC Name:

Diphenyl 6,9,11,14-tetramethyl-7,13-diphenyl-4,10,16-trioxa-7,13-diphosphanonadecane-2,18-diyl bis(6,10,14-trimethyl-8,16,16-triphenoxy-4,7,9,12,15-pentaoxa-8,16-diphosphahexadecan-2-yl) bis(phosphite )

impurity 1

Reference substance name:

Triphenyl phosphite

EC Number:

202-908-4

EC Name:

Triphenyl phosphite

Cas Number:

101-02-0

Molecular formula:

C18H15O3P

IUPAC Name:

Phosphorous acid, triphenyl ester

impurity 2

Reference substance name:

Phenol

EC Number:

203-632-7

EC Name:

Phenol

Cas Number:

108-95-2

Molecular formula:

C6H6O

IUPAC Name:

phenol

Specific details on test material used for the study:

TEST MATERIAL NAME (as stated in study report): D 25-207 (ID Code TKA 40108)

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. #06520471
- Expiration date of the lot/batch: November 1996
- Purity: > 95%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Stock solutions were prepared in water. The stock solutions to produce the desired test concentrations were pumped into a continuous flow of water (3.75 1/h) by high precision positive displacement pumps. After 6 hours of constant operation of the dosing system the fish were transfered into the aquaria.

Test organisms

Test organisms (species):

other: Brachydanio rerio

Details on test organisms:

TEST ORGANISM
- Common name: Zebra-Fish
- Source: P. Hohler / CH-4314 Zeiningen
- Length at study initiation: 35 mm (30 - 38 mm) based on 7 fish
- Weight at study initiation: 0.35 g (0.19 - 0.51 g) based on 7 fish

ACCLIMATION
- Acclimation period: 24 hours
- Feeding frequency during acclimation: none

FEEDING DURING TEST
- Frequency: none

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

180 mg CaCO3/L

Test temperature:

23 ± 1°C

Nominal and measured concentrations:

Nominal: 10,18, 32, 58,100 mg test substance/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 liters filled with 15 liters (35/22/26 cm).
- Aeration: Gentle aeration after 48 hours exposure
- Type of flow-through: precision positive displacement pumps
- No. of organisms per vessel: seven
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): one
- Biomass loading rate: 0.03 g/L in 24 h (flow rate 3.75 L/h)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (carbon filter)

OTHER TEST CONDITIONS
- Photoperiod: Fluorescent light, 16 hours daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality was recorded after 2-4, 24, 48, 72 and 96 hours exposure. The fish were examined daily for symptoms.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

101 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

58 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Mortality occured only in the highest concentration of 100 mg/I (43% mortality). Fish showed sublethal effects at concentrations > 58 mg/1 (loss of equilibrium, change in the swimming behaviour, respiratory movement and pigmentation).

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

In a guildeline (92/69/EEC C.1) flow-through study, the 96-hour LC50 for D 25-207 in Zebra-Fish was 101 mg/L (nominal conc.). The NOEC was 58 mg/L. Water solubility of the test substance was indicated to be < 100 mg/L.