Poly (dipropyleneglycol) Phenyl phosphite

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Version / remarks:

92/69/EEC C.3

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]

EC Number:

279-499-4

EC Name:

Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]

Cas Number:

80584-86-7

Molecular formula:

C102H134O31P8

IUPAC Name:

Diphenyl 6,9,11,14-tetramethyl-7,13-diphenyl-4,10,16-trioxa-7,13-diphosphanonadecane-2,18-diyl bis(6,10,14-trimethyl-8,16,16-triphenoxy-4,7,9,12,15-pentaoxa-8,16-diphosphahexadecan-2-yl) bis(phosphite )

impurity 1

Reference substance name:

Triphenyl phosphite

EC Number:

202-908-4

EC Name:

Triphenyl phosphite

Cas Number:

101-02-0

Molecular formula:

C18H15O3P

IUPAC Name:

Phosphorous acid, triphenyl ester

impurity 2

Reference substance name:

Phenol

EC Number:

203-632-7

EC Name:

Phenol

Cas Number:

108-95-2

Molecular formula:

C6H6O

IUPAC Name:

phenol

Specific details on test material used for the study:

TEST MATERIAL NAME (as stated in study report): D 25-207 (ID Code TKA 40108)

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. #06520471
- Expiration date of the lot/batch: November 1996
- Purity: > 95%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Stock solution: 100.7 mg test substance were mixed with water by polytron for 5 minutes and made up to 1000 ml with water. Calculated amounts of this stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed . The test Solutions were equilibrated for 24 hours under test conditions. The algae were then transferred into the flasks.

Test organisms

Test organisms (species):

other: Selenastrum capriconotum

Details on test organisms:

TEST ORGANISM
- Common name: Green algae
- Source (laboratory, culture collection): Collection of algae cultures, Pflanzenphysiologisches Institut University, Nikolausberger Weg 18, D-37073 Göttingen, Germany
- Method of cultivation: per guideline

ACCLIMATION
- Acclimation period: 3 days under test conditions

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

24 ± 1°C

Nominal and measured concentrations:

Nominal: 10, 18, 32, 58 and 100 mg test substance/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml Erlenmeyer flasks, cotton Stoppers, on Lab shaker,
- Material, size, headspace, fill volume: 50 mL test solution per flask.
- Initial cells density: 10300 cells/mL
- No. of vessels per concentration (replicates): three
- No. of vessels per control (replicates): six

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Composition according to the guideline

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Cell densities were measured at 24, 48 and 72 hours exposure on a "TOA" cell counter.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

other: ErC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

other: EbC50

Effect conc.:

36 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Details on results:

At the highest test concentration of 100 test substance/1 only 41.8% inhibition (calculated on growth rate) was found, therefore the ErC50 was greater than 100 mg/1.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

In a guildeline (92/69/EEC C.3) study, the 72-hour ErC50 for D 25-207 in Green algea was >100 mg/L (nominal conc.). At the highest test concentration of 100 test substance/L 41.8% inhibition (calculated on growth rate) was found. Water solubility of the test substance was indicated to be < 100 mg/L.