Poly (dipropyleneglycol) Phenyl phosphite

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

Version / remarks:

87/302/EEC page 118-122

Deviations:

yes

Remarks:

Instead of a centrifuged sludge a settled sludge was used.

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

OECD Guideline 209,1984

Deviations:

yes

Remarks:

Instead of a centrifuged sludge a settled sludge was used.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]

EC Number:

279-499-4

EC Name:

Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]

Cas Number:

80584-86-7

Molecular formula:

C102H134O31P8

IUPAC Name:

Diphenyl 6,9,11,14-tetramethyl-7,13-diphenyl-4,10,16-trioxa-7,13-diphosphanonadecane-2,18-diyl bis(6,10,14-trimethyl-8,16,16-triphenoxy-4,7,9,12,15-pentaoxa-8,16-diphosphahexadecan-2-yl) bis(phosphite )

impurity 1

Reference substance name:

Triphenyl phosphite

EC Number:

202-908-4

EC Name:

Triphenyl phosphite

Cas Number:

101-02-0

Molecular formula:

C18H15O3P

IUPAC Name:

Phosphorous acid, triphenyl ester

impurity 2

Reference substance name:

Phenol

EC Number:

203-632-7

EC Name:

Phenol

Cas Number:

108-95-2

Molecular formula:

C6H6O

IUPAC Name:

phenol

Specific details on test material used for the study:

TEST MATERIAL NAME (as stated in study report): D 25-207 (ID Code TKA 40108)

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. #06520471
- Expiration date of the lot/batch: November 1996
- Purity: > 95%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Stock solutions were prepared in dechlorinated drinking water.

Test organisms

Test organisms (species):

other: Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 06/02/95.

Details on inoculum:

Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 06/02/95. The pH after collection was 7.6. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. The pH of the sludge before use was 8.0.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

20 ± 2 deg C

Nominal and measured concentrations:

Nominal: 100, 32.0,10.0, 3.2 and 1.0 mg/L

Details on test conditions:

The sludge concentration in the test bottles was 1.49 g/1 (dry weight). 6.4 ml of nutrient solution , 74 ml water, 40 ml of the test solutions and 80 ml of the sludge were mixed in 250 ml BOD flasks and aerated immediately.

Measurements: Oxygen consumption per hour in mg/liter with a ORION - Electrode Type 97-08, ORION Microprocessor lonalizer 901 and plotted on a recorder.

Calculations: Inhibition was calculated on the basis of the measured time dependent oxygen consumption of a Blank and Test solution. Results ( ECxx Values ) were calculated with linear regression.

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol, 32.0,10.0 and 3.2 mg/L

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

EC50(3h) = 16.3 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

In a guideline (OECD 209) activated sludge respiratory inhibition test, the EC50 for D 25-207 was > 100 mg/L (nominal conc.). Water solubility of the test substance was indicated to be < 100 mg/L.