Poly (dipropyleneglycol) Phenyl phosphite

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

92/69/EEC C.2

Deviations:

yes

Remarks:

No analytical determination of test substance concentrations

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]

EC Number:

279-499-4

EC Name:

Oxybispropylenebis[(1,5,9,13,17,21-hexamethyl-7,15,23,23-tetraphenoxy-3,6,8,11,14,16,19,22-octaoxa-7,15,23-triphosphatricos-1-yl)(phenyl)phosphine]

Cas Number:

80584-86-7

Molecular formula:

C102H134O31P8

IUPAC Name:

Diphenyl 6,9,11,14-tetramethyl-7,13-diphenyl-4,10,16-trioxa-7,13-diphosphanonadecane-2,18-diyl bis(6,10,14-trimethyl-8,16,16-triphenoxy-4,7,9,12,15-pentaoxa-8,16-diphosphahexadecan-2-yl) bis(phosphite )

impurity 1

Reference substance name:

Triphenyl phosphite

EC Number:

202-908-4

EC Name:

Triphenyl phosphite

Cas Number:

101-02-0

Molecular formula:

C18H15O3P

IUPAC Name:

Phosphorous acid, triphenyl ester

impurity 2

Reference substance name:

Phenol

EC Number:

203-632-7

EC Name:

Phenol

Cas Number:

108-95-2

Molecular formula:

C6H6O

IUPAC Name:

phenol

Specific details on test material used for the study:

TEST MATERIAL NAME (as stated in study report): D 25-207 (ID Code TKA 40108)

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. #06520471
- Expiration date of the lot/batch: November 1996
- Purity: > 95%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Stock solution: 202.4 mg of the test substance were mixed with 800 ml water by polytron for 5 minutes, homogenized by ultrasonification for 3 minutes and made up to 2000 ml with water. Calculated amounts of this stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed. After an equilibration time of 24 hours the daphnia were transferred into the beakers.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus 1820
- Source: in house
- Feeding during test: none

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: 24 hours before the begin of the exposure reproductive
daphnia are separated from the young by sieving all individuals through a 800 µm sieve. This Operation is repeated immediately before the exposure and the young (0-24h of age) are retained for the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

270 mg CaCO3/L

Test temperature:

20±1°C

Nominal and measured concentrations:

Nominal: 10,18,32,58 and 100 mg test substance/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers covered with watch glasses
- Material, size, headspace, fill volume: 100 ml solution per beaker
- Aeration: none
- No. of organisms per vessel: five
- No. of vessels per concentration (replicates): four
- No. of vessels per control (replicates): four

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water

OTHER TEST CONDITIONS
- Photoperiod: Fluorescent light, 16 hours daily
- Light intensity: approx. 1500 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilizations were recorded after 24 and 48 hours exposure.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

immobile

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Daphnids were less mobile at test concentrations 18, 32, 58 and 100 mg/1 compared to control. 5 daphnids were immobilized at test concentrations 100 mg/1, all other concentrations no animals were immobilized.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

In a guildeline (92/69/EEC C.2) study, the 48-hour EC50 for D 25-207 in Daphnia magna was >100 mg/L (nominal conc.). The NOEC based on reduced mobility was 10 mg/L. Water solubility of the test substance was indicated to be < 100 mg/L.