4,4'-oxydi(benzenesulphonohydrazide)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

NA

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

no data

Vehicle:

yes

Details on test solutions:

Stock solution was prepared by diluting test chemical in dimethylformamide (200,000 mg/L). Test solutions were prepared by adding the corresponding amounts of stock solution into 5 L of dilution water. The final concentration of solvent was 100 µL/L.

Test organisms (species):

Oryzias latipes

Details on test organisms:

Age: about 1 year
Length: 2.29 cm (2.19 ~ 2.40 cm)
Weight: 0.197 g (0.161 ~ 0.229 g)
Loading: 5.0 L aquarium per 10 fish

Acclimation
Acclimation period: Fish were acclimated to the test condition for 14 days before the test.
Type and amount of food: Feed was supplied to fish until 24 hrs before the test but not during the test.
Supplier: Tsunashima Fishing Ltd.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

no

Hardness:

NA

Test temperature:

24 ± 1 °C

pH:

7.4-7.7

Dissolved oxygen:

6.7-8.5 mg/L

Salinity:

NA

Nominal and measured concentrations:

please refer to results

Details on test conditions:

Dilution water source: Dechlorinated and air-saturated tap water passed through activated carbon
Water chemistry: DO = 6.7 ~ 8.5 mg/L, pH = 7.4 ~ 7.7
Temperature: 24 ± 1 °C
Light dark cycle: 16/8 (light/dark)
Test solution was renewed every 24 hrs.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 6.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: no mortality was observed in the control groups

Results with reference substance (positive control):

not reported

Sublethal observations / clinical signs:

Meassured test concentrations compared to nominal concentrations during the semistatic test

Nominal Concentration (mg/L)	Measured Concentration				Mean Measured Concentration*
	0 hr (New)		24 hrs (Old)
	(mg/L)	(% of Nominal)	(mg/L)	(% of Nominal)	(mg/L)	(% of Nominal)
Control	< 0.02	–	< 0.02	–	–	–
Solvent Control	< 0.02	–	< 0.02	–	–	–
20.0	18.9	95	2.29	11	6.6	33

Results on acute toxicity to Oryzias latipes

Exposure time	24 hrs	48 hrs	72 hrs	96hrs
LC50	> 6.6	> 6.6	> 6.6	> 6.6
95% confidence limits	–	–	–	–
Highest test concentration resulting in 0% mortality	> 6.6	> 6.6	> 6.6	> 6.6
Lowest test concentration resulting in 100% mortality	> 6.6	> 6.6	> 6.6	> 6.6

Cumulative mortality of Oryzias latipes

Nominal Concentration (mg/L)	Mean Measured Concentration (mg/L)	Number of Organisms tested	Cumulative Number of DeadFish
			24 hrs	48 hrs	72 hrs	96 hrs
Control	–	10	0	0	0	0
Solvent control	–	10	0	0	0	0
20.0	6.6	10	0	0	0	0

Validity criteria fulfilled:

not specified

Conclusions:

The 96 hr-LC50 of OBSH to Oryzias latipes was > 6.6 mg/L.
No toxic symptom and dead fish were observed in the control and solvent control.

Executive summary:

The acute toxicity of OBSH to fish was tested applying the OECD Guideline no. 203. The test was conducted with the fish Oryzias latipes under semi static conditions. Test media was renewed every 24 hours and fish were not fed during the study. No toxic symptom and dead fish were observed in the control and solvent control during teh study. The 96 hr-LC₅₀(α=0.05) could not be determined by statistical method because the mortality at the maximum concentration was less than 50 %. LC50 is therefore reported as >6.6 mg/L (Meassured concentration, HPLC method)

Description of key information

A reliable short-term (96 hr, semi-static) toxicity study to fish was carried out according to OECD 203, resulting in an 96h-LC50 of >6.6 mg/L (mean measured).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

6.6 mg/L