4,4'-oxydi(benzenesulphonohydrazide)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

22 July, 2010

Deviations:

no

Principles of method if other than guideline:

not relevant

GLP compliance:

yes

Specific details on test material used for the study:

Identity: 4,4’-Oxybis(benzenesulfonyl hydrazide) (OBSH)
CAS No.: 80-51-3
EC No.: 201-286-1
KIT Code No.: K-5450
Lot No.: 201512001
Appearance: White fine powder
Purity: 99.5~100%
Expiry date: December, 2016
Storage conditions: Room temperature / Protect from moisture and light

Analytical monitoring:

yes

Details on sampling:

The concentrations at the top, middle and bottom of the stock solution were 45, 45 and 46 mg/L, respectively and CV(%) were 2.2%. This result showed that there was no difference between the point of measurements, and the solution was identified as being very homogeneous
After 30 minutes of aeration, the contents of the first vessel were transferred to a BOD bottle and the respiration rate was measured over a period of up to 10 minutes. Dissolved oxygen readings were recorded every 10 seconds for 10 minutes using a Multi 3430 meter (WTW, Germany).

Vehicle:

yes

Details on test solutions:

to be decided

Test organisms (species):

activated sludge

Details on inoculum:

Activated sludge was collected from the Sewage Treatment Plant, Munsan, Jinju on November 15, 2016. After collection of activated sludge, it was allowed to settle for ca.10 minutes to remove the coarse particles and was sieved using a testing sieve (aperture: 1.00mm). The sludge was centrifuged for 10 minutes at ca.10,000 m/s2 (ca.1,030 g) and the supernatant liquid was discarded. The sludge was re-suspended in deionized water, with shaking, and the wash-water was then removed. The total suspended solids concentration of activated sludge was determined to be 3,150 mg/L by using a moisture analyzer, and the activated sludge was continuously aerated at the test temperature prior to use.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Remarks on exposure duration:

If the test substance is rapidly degraded e.g. abiotically via hydrolysis, and the concentration cannot be adequately maintained, a shorter exposure period can be used. Thus the exposure period was reduced to 30 minutes

Post exposure observation period:

no

Hardness:

No data

Test temperature:

20.4 ±0.2 oC

pH:

7.5 -7.6

Dissolved oxygen:

above 60-70%

Salinity:

NA

Conductivity:

NA

Nominal and measured concentrations:

to be decided

Details on test conditions:

Please refer to information stated in the section above

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

30 min

Dose descriptor:

NOEC

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

>= 5 - <= 20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

The inhibition of 4,4’-Oxybis(benzenesulfonyl hydrazide) (OBSH) at 1, 5 and 20 mg/L were negative values (percentage). EC50 of 4,4’-Oxybis(benzenesulfonyl hydrazide) (OBSH) is therefore reproted as higher than 20 mg/L.

Results with reference substance (positive control):

The results obtained with the reference item 3,5-dichlorophenol, showed an EC50 of 5.5 mg/L. The EC50was within the 2 to 25 mg/L range, and it was considered acceptable for the test

No data

Validity criteria fulfilled:

yes

Conclusions:

30 min EC50 > 20 mg/L (respiration rate)
30 min NOEC = 5 mg/L (respiration rate)
30 min EC10 =5-20 mg/L (respiration rate)

Executive summary:

The test item was tested according to OECD Guideline no 209: Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation). The study was conducted according to GLP. Resulting NOEC = 5 mg/L EC10 = 5 -20 mg/L and EC50> 20 mg/L based on the endpoint total respiration.

Description of key information

A reliable toxicity study (3h) to microrganisms was carried out according to OECD 209, resulting in an 3h-EC50 (inhibition of total respiration) of > 20 mg/L (nominal) and a NOEC (inhibition of total respiration) of 5 mg/L (nominal).

Key value for chemical safety assessment

EC50 for microorganisms:

20 mg/L

EC10 or NOEC for microorganisms:

5 mg/L