Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 629-720-9 | CAS number: 1219826-66-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Justification for type of information:
- For details and justification of read-across please refer to the report attached in section 13 of IUCLID.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.431 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: (0.346 - 0.538)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1.05 mg/L
- Remarks on result:
- other: IUCLID4 note: "m" (measured/nominal)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.18 mg/L
- Remarks on result:
- other: IUCLID4 note: "m" (measured/nominal)
- Details on results:
- The LC50 (96 h) was calculated to be 0.431 mg/L with 95% confidence limits of 0.346 and 0.538 m/L. The highest concentration causing no mortality (no observed effect concentration, NOEC) after 96 h amounted to 0.18 mg/L whereas 0.32 m/L was the lowest tested concentration (LOEC,96 h) at which lethality was observed as compared to the control. The LC100 was 1.05 m/L, which was observed after 29 hours.
- Reported statistics and error estimates:
- The LC50 was calculated using the computer program Toxcalc v 5.0 applying the trimmed Spearman-Karber method
- Sublethal observations / clinical signs:
- RESULTS: EXPOSED
- Nominal/measured concentrations: 0, 0.1, 0.18, 0.32, 0.58,1.05 mg/L (nominal)
- Effect data (Mortality):
24h 48h 72h 96h
Control 0 0 0 0
0.1 0 0 0 0
0.18 0 0 0 0
0.32 0 0 0 1
0.58 0* 1* 5 6
1.05 1** 7
* reduced acitivity
** very reduced activity
For ethical reasons two fish were euthanised at the
concentration of 0.58 ml/L after 53 hours.
The LC50 (96 h) was calculated to be 0.431 mg/L with 95% confidence limits of 0.346 and 0.538 m/L. The highest
concentration causing no mortality (no observed effect concentration, NOEC) after 96 h amounted to 0.18 mg/L
whereas 0.32 m/L was the lowest tested concentration (LOEC,96 h) at which lethality was observed as compared to the
control. The LC100 was 1.05 m/L, which was observed after 29 hours.
- Concentration / response curve: -
- Effect concentration vs. test substance solubility: -
- Other effects: see mortality data * and **
RESULTS: CONTROL
- Number/percentage of animals showing adverse effects: None
- Nature of adverse effects: - - Validity criteria fulfilled:
- yes
- Conclusions:
- Validity criteria can be considered as fulfilled.
Mortality of control animals was <10%, the concentration of dissolved oxygen in all test vessels was > 60% saturation and the concentration of test substance >= 80% of initial concentration during test.
Although quantification of the applied concentrations was not possible due to the absence of a suitable analytical method it was possible to analyze the two stock solutions used to prepare the test concentrations. These analyses showed that the stock solutions were stable even after storage for almost 7 months in the refrigerator. Considering the high water solubility of the test substance and its stability it is reasonable to assume that the actual concentrations were identical to the nominal concentrations during the entire test. A dose-response related increase in mortality was observed. Study peformed according to standard protocol and under GLP. - Executive summary:
The acute toxicity of Triameen Y12D to the fresh water fish Brachydanio rerio (zebra fish) was tested under semi-static conditions in accordance with OECD and EEC guidelines for testing of chemicals. The test was performed in compliance with Good Laboratory Practice (GLP). The fish were exposed to the following nominal test concentrations: 0.1- 0.18 – 0.32 – 0.58 and 1.05 mg/l. The LC50(96 h) was calculated to be 0.43 mg/l with 95% confidence limits of 0.346 and 0.538 mg/l. The highest concentration causing no mortality (no observed effect concentration, NOEC) after 96 h amounted to 0.18 mg/l whereas 0.32 mg/l was the lowest tested concentration (LOEC, 96 h) at which lethality was observed as compared to the control. The LC100was 1.05 mg/l, which was observed after 29 hours.
At the concentrations where lethality was established, sublethal effects, such as reduced activity, deviations in the behavior or the appearance of the fish, were observed. For ethical reasons two fish were euthanised at the concentration of 0.58 mg/l after 53 hours.
Quantification of the applied concentrations was not possible due to the absence of a suitable analytical method. The currently available analytical method is capable of analyzing down to only 10 mg/l. Therefore only the two stock solutions used to prepare the test concentrations were analyzed. These analyses showed that the stock solutions were stable even after storage for almost 7 months in the refrigerator. All concentrations mentioned in this report are therefore based on nominal concentrations. Considering the high water solubility of the test substance and its stability it is reasonable to assume that the actual concentrations were identical to the nominal concentrations during the entire test.
This information is used in a read-across approach in the assessment of the target substance. For justification of read-across please refer to the attached read-across report (see IUCLID section 13).
Reference
Description of key information
No short-term fish data are available for branched triamine C16 -18 but the fish data for the branched triamine C12 will be used as a worst-case.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 431 µg/L
Additional information
No short-term fish data are available for branched triamine C16 -18 but the fish data for the branched triamine C12 will be used as a worst-case. Figures for branched triamine C12 (CAS no 2372-82-9) were obtained by performing ecotoxicity tests in reconstituted lab water and are therefore considered as data obtained under extreme worst-case conditions. From these figures it is clear that fish are clearly less sensitive compared to algae and daphnia.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.