Amines, N-(3-aminopropyl)-N-(C16-18 and C18-unsatd. alkyl)trimethylenedi-

Sediment toxicity

Administrative data

Endpoint:

sediment toxicity: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OSPAR Commission (2006) Part A Guideline: a sediment bioassay using an amphipod Corophium sp.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine

EC Number:

219-145-8

EC Name:

N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine

Cas Number:

2372-82-9

Molecular formula:

C18H41N3

IUPAC Name:

N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine

Test material form:

liquid

Remarks:

Yellow

Details on test material:

- Batch/lot no.: 670HC007
- Expiry date: 17.03.2017
- 99.9% purity

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on sediment and application:

Sediment for use in this toxicity test was collected from a point adjacent to the site of Corophium collection. On return to the laboratory, the sediment was wet-sieved through 0.6mm mesh to remove larger infaunal organisms and Corophium within the test size range, and allowed to settle in tall polyethylene containers. After settling, the supernatant water was decanted and the sediment stored in a cool environment at approximately 2-10 °C, until required for testing.
Particle size analysis characterised the sediment as well-sorted, fine sand with a silt/clay content of 5.40% by weight. Median particle diameter was 72.1 μm. The organic material content was calculated from weight loss on ignition to be 8.14%.
Immediately before the initiation of the test, the sediment was thoroughly homogenised and a representative sample taken for wet and dry weight determination. The ratio of wet weight to dry weight was subsequently used to convert nominal exposure concentrations on a wet weight basis to nominal exposure concentrations on a dry weight basis.

Test organisms

Test organisms (species):

Corophium volutator

Details on test organisms:

The test population of C. volutator were collected from the Oyce of Quindry, South Ronaldsay, Orkney (Latitude 58°49'07.1"N, Longitude 2°58'31.1"W). This location was chosen because of its high natural densities of Corophium and, in accordance with the guidance document ‘Biological Effects of Contaminants; Corophium sp. sediment bioassay and toxicity test’, Roddie & Thain (2001). Specimens were gently sieved from their native sediment and held in plastic containers (approximately 5litre capacity) containing some ambient water, with a small amount of detritus, until transfer to the laboratory.
On return to the laboratory, the Corophium were transferred in ambient water to polythene tanks of approximately 200 litre capacity, gentle aeration was supplied. Initial parameters of the holding water were recorded upon arrival at the Fjords Processing Environmental laboratory. The tanks will be maintained at 15±2°C. The holding tank was gradually acclimated from the ambient salinity of less than 5‰ to that of undiluted seawater (approximately 35‰) in increments of approximately 10‰ per day. Once acclimation was complete, the holding tank was maintained under semi-static conditions until test commencement. The holding period in the laboratory is between 3 and 14 days. A small amount of detrital material is retained to provide food and some bottom cover, but not of a density that prevents daily observation of mortality and morbidity. A control chamber was set up to monitor holding tank deaths. If high mortality was observed e.g. >10%, a new batch of Corophium were collected. Specimens of approximately 5mm in body length (excluding rostrum) were used in the toxicity test.

Study design

Study type:

laboratory study

Test type:

static

Water media type:

saltwater

Type of sediment:

natural sediment

Limit test:

no

Exposure duration

Duration:

10 d

Exposure phase:

total exposure duration

Post exposure observation period:

None

Test conditions

Test temperature:

14.5-16.8

pH:

7.70-8.32

Dissolved oxygen:

95.5-99.9% saturation

Salinity:

0 h = 33.5-33.8 During the test = 33.5-36.3

Nominal and measured concentrations:

- Nominal: 0, 10, 100, 320, 1000 and 10000 mg/kg sediment dw

Details on test conditions:

Tests were conducted in 1 litre capacity glass beakers each containing 2 cm depth (approximately 150 g) of amended sediment and 850 ml of overlying seawater (1μm filtered ultra violet treated seawater). Test beakers were maintained in a controlled temperature room for a test period of 10 days.
Three replicates were prepared for each test concentration; controls are replicated five times. The beakers were assigned positions within the test area, arranged in rows of three to five and spaced to maintain effective separation of different treatments. Each row was covered with a rectangular sheet of perspex perforated with a small hole above the centre of each beaker. Aeration was provided and a stream of air bubbles was released at a depth of approximately 6cm from the water surface.
Twenty animals were added to receiving vessels in two groups of ten and then dispensed into a test vessel. Sixty organisms were exposed in total per concentration and one hundred in five vessels for controls. The final level of water in each test vessel was marked with a permanent marker. Salinity is maintained at ±4‰ from the initial 0h figure during the test. A 5 mm drop in water level is equivalent to ~2‰ increase in salinity. Any visible signs of animals on the sediment surface whether alive or dead were recorded.
At the end of the test period, the contents of each beaker were gently sieved through a 0.6mm mesh, and all surviving animals separated and counted. Missing animals were counted as dead; early mortalities will either have been consumed by survivors or have decomposed during the test period.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

10 d

Dose descriptor:

LC50

Effect conc.:

304 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Key result

Duration:

10 d

Dose descriptor:

NOEC

Effect conc.:

127.14 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Reported statistics and error estimates:

The observations recorded from the test vessels at the end of the 10 day experimental period were input into CETIS statistical programme to determine the 10 day LC50 value with 95% confidence limits and also the LC90 value and NOEC.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the 10 d LC50 was equivalent to 304 mg/kg dw and the 10 d NOEC was 127.14 mg/kg dw.

Executive summary:

A study was conducted to determine the toxicity of the test substance to the marine crustacean Corophium volutator according to OSPARCOM Part A Method, in compliance with GLP. Tests were conducted in 1 L glass beakers, each containing 2 cm depth (approximately 150 g) of amended natural sediment and 850 ml of overlying seawater. Three replicates were prepared for each test concentration; controls were replicated five times. Twenty animals, collected from seawater, were added to receiving vessels in two groups of ten and then dispensed into a test vessel. Sixty organisms were exposed in total per concentration and one hundred in five vessels for controls, for a duration of 10 d. The test substance was applied in seawater to the test vessels at concentrations of 0, 10, 100, 320, 1000 and 10000 mg/kg sediment dw before addition of the test organisms. No analytical dose verification was conducted. Under the study conditions, the 10 d LC50 was equivalent to 304 mg/kg dw and the 10 d NOEC was 127.14 mg/kg dw (Hudson, 2015).