Amines, N-(3-aminopropyl)-N-(C16-18 and C18-unsatd. alkyl)trimethylenedi-

Ecotoxicological Summary

Hazard for aquatic organisms

Freshwater

Hazard assessment conclusion:

PNEC aqua (freshwater)

PNEC value:

0.006 mg/L

Assessment factor:

50

Extrapolation method:

assessment factor

PNEC freshwater (intermittent releases):

0.003 mg/L

Marine water

Hazard assessment conclusion:

PNEC aqua (marine water)

PNEC value:

0.001 mg/L

Assessment factor:

500

Extrapolation method:

assessment factor

STP

Hazard assessment conclusion:

PNEC STP

PNEC value:

1.7 mg/L

Assessment factor:

10

Extrapolation method:

assessment factor

Sediment (freshwater)

Hazard assessment conclusion:

PNEC sediment (freshwater)

PNEC value:

3.2 mg/kg sediment dw

Assessment factor:

100

Extrapolation method:

assessment factor

Sediment (marine water)

Hazard assessment conclusion:

PNEC sediment (marine water)

PNEC value:

0.13 mg/kg sediment dw

Assessment factor:

1 000

Extrapolation method:

assessment factor

Hazard for air

Air

Hazard assessment conclusion:

no hazard identified

Hazard for terrestrial organisms

Soil

Hazard assessment conclusion:

no hazard identified

Hazard for predators

Secondary poisoning

Hazard assessment conclusion:

no potential for bioaccumulation

Conclusion on classification

For classification purposes ecotoxicity, biodegradability and bioconcentration have to be considered.

Ecotoxicity

Due to intrinsic properties of amine containing cationic surfactants, river water ecotoxicity tests deliver more reproducible test results with limited uncertainty. As river water has a mitigating effect on ecotoxicity due to sorption of the amines to and suspended matter, a factor of 10 should be applied to the L(E)C₅₀ to correct for the lower ecotoxicity observed. In the absence of sufficient acute daphnia data the 21 d EC₅₀ for daphnia is used as basis for the acute classification of the polyamines as a worst-case. Some test results for tests performed in reconstituted lab water are available and these endpoints can therefore directly be used for classification purposes. The only acute fish and daphnia data available are for N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine (CAS 2372-82-9). For fish a 96 h LC50 of 0.431 mg/L was observed and for daphnia a 48 h EC50 of 0.0775 mg/L.

 

Table   Available algae, daphnia and fish test results (Klimisch 1, 2) for the polyamines.

Polyamines	New CAS number	Old CAS number	72 h algae ERC50(µg/L)	72 h algae ERC10/ NOEC (µg/L)	21 d daphnia EC50 (µg/L)	21 d daphnia EC10/ NOEC (µg/L)	EC50corr(µg/L) (corrected for Classification with Factor 10)
N-(3-aminopropyl)-N- (C12-18 even numbered) alkyl-propane-1,3-diamine	1219458-12-4	91771-18-5	223	137	342	63.7	34
(Z)-N-(3-aminopropyl)-N'-9-octadecenylpropane-1,3-diamine	28872-01-7	28872-01-7	1090	518	684	810	68
N-(3-aminopropyl)-N'-C16-18 (evennumbered), C18 unsaturated alkyl -propane-1,3-diamine	1219458-14-6	61791-57-9					68
(Z)-N-(3-aminopropyl)-N'-[3-(9-octadecenylamino)propyl]propane-1,3-diamine	67228-83-5	67228-83-5					68
N-(3-aminopropyl)-N'-[3-(C16-18 (evennumbered), C18 unsaturated alkyl amino)propyl]propane-1,3-diamine	1219458-11-3	68911-79-5	2470	1270	974	800	97
N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine	2372-82-9	2372-82-9	15	9.5	34	24	15
N-(3-aminopropyl)-N-tallow alkyltrimethylenediamine	85632-63-9	85632-63-9	914	347	566	320	56.6

Figures given in bold are based on read across; figures given in italic and underlined are performed in reconstituted lab water and these are therefore not corrected.

 

 Biodegradability

Triamines

Only valid ready biodegradability test result for linear and branched triamines is available because these substances are very toxic to micro-organisms. Nevertheless all aspects important for achieving a ready biodegradability test result i. e.
1) ultimate (complete) biodegradation (demonstrated in (S) CAS tests, through read across with triamine C12-18 and branched triamine C12 and intermediates)
2) high rates of degradation (through read across with triamine C12-18/branched triamine C12) and
3) occurrence of competent micro-organisms in unacclimated ecosystems (through read across with triamine C12-18) are fulfilled. 
All alkyl linear and branched dipropylene triamines should therefore be classified as readily biodegradable. 

Table Typical members of the family of alkyl dipropylene triamine. 

Chemical name		CAS (old)
Tallow dipropylene triamine	61791-57-9
Cocodipropylene triamine	91771-18-5
Oleyl dipropylene triamine	28872-01-7
N-(3-aminopropyl) -N-dodecylpropane-1,3, diamine	2372-82-9
N-(3-aminopropyl)-N-tallow alkyltrimethylenediamine	85632-63-9

 Compositions of coco and tallow-alkyl chains are specified by Karleskind (1996).

 

Bioconcentration factor

No measured BCF in fish is available. Standard OECD 305 tests are technically very complicated with these strongly sorbing and completely biodegradable substances. Two valid log Kow values are provided in the dossier. One for triamine C12-18 (CAS 1219458-12-4) and one for tetramine oleyl (CAS 67228-83-5). For both a log Kow of -0.3 was observed using the slow stirring method (OECD 123) at pH 6. This log Kow value of -0.3 indicates a low bioaccumulation potential. With this log Kow, a BCF of 3.162 L/kg is calculated using BCFBAF v3.0 estimation program (EPIweb v4.0).

 

Classification according(Classification, Labeling & Packaging Directive 1272/2008/EC)

As all acute ecotoxicity values (corrected and uncorrected) are below 1 mg/L and the chronic data from the chronic daphnia is < 0.1 mg/L (when corrected for classification with factor 10) both chronic and acute classification applies.

This leads to an environmental classification for linear and branched triamines:

- Acute (short-term) aquatic hazard Acute Category 1

- Chronic (long-term) aquatic hazard Chronic Category 2