Hydroxy(2-methylprop-2-enoato-O)zinc

Toxicity to reproduction

Administrative data

Endpoint:

fertility, other

Remarks:

based on test type (migrated information)

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1986

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Males rats were fed diet supplemented with the test material for specific period and then mated with normal females. Males were sacrificed after mating and effect on sperm motility/viability and zinc concentration in reproductive organs observed. Females were allowed to have full term gestation and effect on conception and litter viability were determined.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Zinc sulphate

EC Number:

231-793-3

EC Name:

Zinc sulphate

Cas Number:

7733-02-0

IUPAC Name:

zinc sulfate

Test material form:

not specified

Details on test material:

- Name of test material (as cited in study report): Zinc sulfate anhydrous

Test animals

Species:

rat

Strain:

other: Charles-Foster

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 162 g
- Diet: Crushed rat feed of Hindustan Lever (India), ad libitum

Administration / exposure

Route of administration:

oral: feed

Vehicle:

unchanged (no vehicle)

Details on exposure:

DIET PREPARATION
- Mixing appropriate amounts with (Type of food): Crushed rat feed

Details on mating procedure:

- M/F ratio per cage: 1:1
- Length of cohabitation: 1 day
- Proof of pregnancy: Sperm in vaginal smear (mating performed only once irrespective of results)

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

30-32 days before mating to males only

Frequency of treatment:

Daily, ad libitum

Details on study schedule:

Following the day of mating, males were sacrificed and sperm was immediately collected from epididymis for motility and viability studies. Reproductive organs were dissected out for estimation of zinc. Mated females were allowed to have full term gestation.

Doses / concentrations

Remarks:

Doses / Concentrations:
4000 ppm zinc as zinc sulfate
Basis:
nominal in diet

No. of animals per sex per dose:

Control group: 15
Treatment group: 18

Control animals:

yes, plain diet

Details on study design:

None

Positive control:

None

Examinations

Parental animals: Observations and examinations:

No data

Oestrous cyclicity (parental animals):

No data

Sperm parameters (parental animals):

Parameters examined in male parents:
- Sperm motility and viability

Litter observations:

PARAMETERS EXAMINED
The following parameters were examined in F1 offspring:
- Number of pups, stillbirths, live births and malformation

Postmortem examinations (parental animals):

SACRIFICE
- Male animals: All animals sacrificed following the day of mating and reproductive organs (testis, epididymis, seminal vesicle and prostate) were dissected out for estimation of zinc
- Maternal animals: Not sacrificed; mated females were allowed to have full term gestation.

Postmortem examinations (offspring):

No data

Statistics:

Fisher's one sided T test

Reproductive indices:

None

Offspring viability indices:

None

Results and discussion

Results: P0 (first parental generation)

General toxicity (P0)

Clinical signs:

not specified

Body weight and weight changes:

not specified

Food consumption and compound intake (if feeding study):

not specified

Organ weight findings including organ / body weight ratios:

not specified

Histopathological findings: non-neoplastic:

not specified

Other effects:

not specified

Reproductive function / performance (P0)

Reproductive function: oestrous cycle:

not specified

Reproductive function: sperm measures:

effects observed, treatment-related

Reproductive performance:

effects observed, treatment-related

Details on results (P0)

REPRODUCTIVE FUNCTION: SPERM MEASURES (PARENTAL ANIMALS): Motility of the sperm was significantly reduced in the zinc treated rats at all time intervals viz. 30 min., 2 h and 4 h. The percentage reduction as compared to the controls was 8.5, 25 .3 and 29 .0 respectively. Sperm viability was unaffected. (See Table 7.8.1/3 for details)

REPRODUCTIVE PERFORMANCE (PARENTAL ANIMALS): Mating by zinc treated males caused significant lowering of incidence of conception and number of live births per mated female. Only 11 females conceived out of 18 mated by zinc treated males whereas all females mated with control rats conceived. (See Table 7.8.1/1 for details)

OTHER FINDINGS (PARENTAL ANIMALS): Zinc content was significantly increased only in the testis (25 %) and sperm (18 %) of the zinc treated rats. (See Table 7.8.1/2 for details)

Effect levels (P0)

1

Dose descriptor:

dose level: 4000 ppm zinc

Based on:

test mat.

Sex:

male

Basis for effect level:

other: overall effects sperm characterization; other: zinc concentration in reproductive organs

Remarks on result:

not measured/tested

Remarks:

Effect level not specified (migrated information)

2

Dose descriptor:

other: Not treated but mated with males fed 4000 ppm zinc

Based on:

test mat.

Sex:

female

Basis for effect level:

other: overall effects incidence of conception

Remarks on result:

not measured/tested

Remarks:

Effect level not specified (migrated information)

Results: F1 generation

General toxicity (F1)

Clinical signs:

no effects observed

Mortality / viability:

no mortality observed

Body weight and weight changes:

not specified

Sexual maturation:

not specified

Organ weight findings including organ / body weight ratios:

not specified

Gross pathological findings:

not specified

Histopathological findings:

not specified

Details on results (F1)

VIABILITY (OFFSPRING): No stillbirth in any of the groups.

OTHER FINDINGS (OFFSPRING): No malformed litter in any of the groups.

Effect levels (F1)

Dose descriptor:

other: Offsprings of 4000 ppm zinc treated males

Generation:

F1

Sex:

male/female

Basis for effect level:

other: overall effects live births; offspring malformation

Remarks on result:

not measured/tested

Remarks:

Effect level not specified (migrated information)

Overall reproductive toxicity

Reproductive effects observed:

not specified

Any other information on results incl. tables

Table 7.8.1/1: Outcome of mating by zinc treated male rats

	Mating males
	Control	Experimental
Number of females mated	15	18
Number of females conceived	15	11
Normal live litter
Total number	101	61
Number / mated female	6.73	3.38*

*Difference was significant at 5% level

Table 7.8.1/2: Zinc content of the reproductive tissues of zinc treated male rats

	Control	Experimental
Testis	28.1± 1.63 (15)	35.3 ± 2.13 (18)*
Epididymis	58.3 ± 3.46 (10)	61.4 ± 6.63 (10)
Seminal vesicle	24.4 ± 3.25 (10	33.8 ± 5.96 (10)
Prostate (whole)	48.8 ± 6.90 (10)	51.7 ± 6.36 (10)
Sperm	559 ± 14.8 (10)	658 ± 24.7 (10)*

Results expressed as Mean ± SEM in µg/g, except for sperm in µg/g dw, for the number of rats shown in parentheses. * P < 0.02

Table 7.8.1/3: Motility and viability of the sperm of zinc treated rats

	Control (15)	Experimental (18)
Motility (Emmen's unit)
30 min	25.9 ± 0.57	23.7 ± 0.47*
2 hours	23.7 ± 0.58	17.7 ± 1.15**
4 hours	17.2 ± 1.10	12.2 ± 1.42*
% viable at 4 hours	55.7 ± 4.80	49.0 ± 4.60

Values expressed as Mean ± SEM for the number of rats shown in parentheses.* P < 0.01 ; **P<0.001

Applicant's summary and conclusion

Conclusions:

Under the test conditions, dietary zinc supplementation at 4000 ppm reduced male fertility in rats.

Executive summary:

A study was conducted to determine the effects of dietary zinc supplementation on male fertility in Charles-Foster rats. 4000 ppm zinc as zinc sulfate was fed to 18 test males in diet for 30-32 days. 15 control males were fed normal diet for the same duration. All animals mated with individual normal females once between Day 30 and 32. After mating, males were sacrificed for sperm characterization and zinc concentration analysis in different reproductive organs. Mated females were allowed to have full term gestation.

Mating by treated males caused significant lowering of incidence of conception and number of live births per mated female. However, no stillbirth or malformed litter was observed. Motility of the sperm was significantly reduced in the treated rats but viability was unaffected. Zinc content was significantly increased only in the testis and sperm of the treated rats.

The results indicate that dietary zinc supplementation at 4000 ppm reduced male fertility in rats under the conditions of the study.