2-Propenoic acid, 2-chloro-, 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl ester

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 Feb - 19 Mar 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all test concentrations (new test solution and old test solution prior to renewal)
- Sampling method: Collected in the middle layer of the vessel

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution I was prepared by dissolving 100 mg test substance in tetrahydrofuran to a final volume of 10 mL. Stock solution II was obtained by taking 1000 µL of stock solution I and adding tetrahydrofuran to a final volume of 10 mL. Stock solution III was prepared individually for each test concentration. 80, 140, 250, 450 and 800 µL, respectively, of stock solution II was taken, and tetrahydrofuran was added to a volume of 10 mL. Between all dilution steps, the solution was mixed by inversion. For the test concentrations, 0.11 mL of the stock solution III was taken and dilution water was added to a final volume of 1.1 L.
- Controls: control with dilution water only and solvent control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): tetrahydrofuran
- Concentration of vehicle in test medium (stock solution and final test solution): 100 µL/L

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: National Institute of Environmental Studies, Japan; received 1995 and maintained in the testing facility
- Age at study initiation (mean and range, SD): juveniles, less than 24 h old
- Feeding during test: none

CULTURE CONDITIONS OF PARENT DAPHNIDS
- Culture period: 28 days or less
- Culture conditions (same as test or not): same as test, but Elendt medium
- Type and amount of food: Chlorella vulgaris, 6 mg C /2 L /day

ACCLIMATION
- Acclimation period: 48 h or more
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Chlorella vulgaris, 6 mg C /2 L /day
- Health during acclimation (any mortality observed): Mortality of parent daphnids during 2 weeks period prior to the start of exposure: 20% or less

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

<= 250 mg CaCO3/L

Test temperature:

20 ± 1 °C

pH:

6.0 - 9.0 (variation < 1.5)

Dissolved oxygen:

3 mg/L or more

Nominal and measured concentrations:

Nominal: 0.00080, 0.0014, 0.0025, 0.0045 and 0.0080 mg/L
Measured (TWA): 0.000383, 0.000640, 0.00102, 0.00164 and 0.00371 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed (ground-in-stopper)
- Material, size, headspace, fill volume: 1 L volumetric bottle, fill volume 100 mL
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated water processed by treating Yokohama city tap water with an activated charcoal filter and sodium thiosulfate
- Total organic carbon: 0.3 mg/L
- Culture medium different from test medium: yes
- Intervals of water quality measurement: at the time of preparation of new solution, immediately prior to renewal and at the end of exposure (temperature, dissolved oxygen, apparence)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 800 lux or less

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): number of immobile daphnids, other abnormal symptoms; after 24 h and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.78
- Range finding study
- Test concentrations: 0.00080 and 0.0080 mg/L
- Results used to determine the conditions for the definitive study: 0 % immobility at both concentrations

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- At the highest concentration two immobile daphnids were observed after 48 h. At the other concentrations or in the controls, no immobility occurred.

Results with reference substance (positive control):

- EC50 (48h) = 0.75 mg/L (95% confidence limits: 0.67 - 1.00 mg/L)
Exposure period: 4 - 6 Jan 2012

Description of key information

Up to the highest attainable concentration no EC50 could be reached.

48h-EC50 > 0.00371 mg/L (OECD 202, Daphnia magna)

Additional information

One study is available on the short-term toxicity of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl 2-chloropropenoic acid ester (CAS 96383-55-0) to aquatic invertebrates. The study was conducted according to the OECD guideline 202 and GLP (Matsumoto, 2012). As the test substance is poorly soluble in water, tetrahydrofuran was used as a solvent for the preparation of the test solutions. The test organism Daphnia magna was exposed under semi-static conditions to nominal test concentrations of 0.00080, 0.0014, 0.0025, 0.0045 and 0.0080 mg/L for 48 hours. At the end of the exposure period, 10% immobilization was observed only at the highest test concentration. The EC50 (48h) was therefore determined to be above the highest test concentration, corresponding to a measured value of > 0.00371 mg/L.