Reaction mass of sodium 1-methoxy-1-oxohexadecane-2-sulphonate and sodium methyl 2-sulphooctadecanoate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 12, 2000- December 10, 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The test solution (1.0-1.5 mL) was collected from each of the testing containers at the beginning of the test and at 24 hours using LC-MS.

Vehicle:

no

Details on test solutions:

A solution containing 150 mg/L of the test material was added to dilution water to prepare test solutions of the pre-determined concentrations in the required amount.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Japanese killifish
- Source: Yatomi Fish Farm, Aichi Prefecture
- Lot number: Meju 010117
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 2.18-2.46 cm
- Weight at study initiation (mean and range, SD): 0.1684-0.2740 g
- Method of breeding: no data
- Feeding during test: none


ACCLIMATION
- Acclimation period: more than 12 days
- Acclimation conditions (same as test or not): same
- Type and amount of food: Tetramine
- Feeding frequency: no data, but no food was given to the killifish 24 hours prior to the start of exposure
- Health during acclimation (any mortality observed): during 7 days prior to start of exposure, mortality was 1%

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None.

Hardness:

25 mg/L as CaCO3

Test temperature:

23.1 - 23.7 ºC

pH:

6.8-7.3

Dissolved oxygen:

5.0-8.3 mg/L

Salinity:

Not applicable.

Nominal and measured concentrations:

The concentration of the test material in the test solution was measured at the beginning of the test and at 24 hours. The initial test material concentration was 0.22-3.83 mg/L, while the concentration at 24 hours was 0.21-3.7 mg/L with the ratios to the nominal concentration (0.26-5.00 mg/L) at 69.8-81.3%. Because the actual measurements were outside of ± 20% of the set concentrations, geometric mean values were used to calculate the test results.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass containers with capacity of 3 L
- Renewal rate of test solution (frequency/flow rate): water renewal after 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water supplied in Nagoya City was filtered through activated charcoal for dechlorination before use.
Hardness 25 mg CaCO3 equivalent
pH: 7.0


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hrs on, 8 hrs off
- Light intensity: room light


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 (nominal conc.: 0.26, 0.48, 0.86, 1.54, 2.78 and 5.00 mg/L
- Range finding study
- Test concentrations: no data
- Results used to determine the conditions for the definitive study: Medial Lethal Concentration for 96-hr exposure was 2.24 mg/L. The highest concentration for 0% mortality and lowest concentration for 100% mortality was 1 mg/L and 5 mg/L, resp.

Reference substance (positive control):

yes

Remarks:

copper (II) sulfate

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 1.13-1.99 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1.13 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1.99 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No abnormal symptoms that suggested toxicity were observed in the control and all study cultures during the study period.

Results with reference substance (positive control):

The LC50-96hr with the reference material was 0.46 mg/L

Reported statistics and error estimates:

Following calculation of the mortality from the number of deaths and the number of the test fish, the LC50 was calculated based on Binominal method, utilizing the calculation method of TOXDAT Multi-Method program developed by the EPA.

Validity criteria fulfilled:

yes

Conclusions:

The 96hr LC50 was determined to be 1.5 mg/L.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

The publication does not give sufficient experimental details to derive the LC50. However, the study is useful in showing the trend of increasing toxicity with increasing alkyl chain lengths.

Qualifier:

according to guideline

Guideline:

other: JIS K0102: Testing methods for Industrial wastewater- acute toxicity test with fish (1991)

Deviations:

not specified

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Oryzias latipes

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

298 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: for C12 α-SFMe

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

24 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: for C14 α-SFMe

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: for C16 α-SFMe

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: For C14/16: 30/70

Validity criteria fulfilled:

not specified

Conclusions:

The fish toxicity of α-SFMe decreased with decreased alkyl chain length.
The LC50 of the combined C14/C16 mix can be estimated from the LC50 of the separate components, using the simple combination toxicology (LC50 calculated 1.82 mg/L).

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

The endpoint of short-term toxicity to fish of the target substance, reaction mass of sodium methyl 2-sulphooctadecanoate and sodium 1-methoxy-1-oxohexadecane-2-sulphonate, was based on the data from the two main components, C16MES and C18MES, and substances similar to the target substance as source chemicals. No definitive value for the reaction mass was derived nor necessary as the data of the two components of the reaction mass are used in separate environmental risk assessments.
Please see the read-across justification in section 13.2

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across: supporting information

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Remarks:

Substance: C16MES (223-676-0)

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.44 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Remarks:

Substance: C18MES (223-770-1)

Basis for effect:

mortality (fish)

Remarks on result:

not measured/tested

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

298 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Substance: C12 α-SFMe

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

24 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Substance: C14 α-SFMe

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Substance: C16 α-SFMe

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Substance: C14/16: 30/70

Basis for effect:

mortality (fish)

Conclusions:

The endpoint of short-term toxicity to fish of the target substance, reaction mass of sodium methyl 2-sulphooctadecanoate and sodium 1-methoxy-1-oxohexadecane-2-sulphonate, was based on the data from the two main components, C16MES and C18MES, and substances similar to the target substance as source chemicals. No definitive value for the reaction mass was derived nor necessary as the data of the two components of the reaction mass are used in separate environmental risk assessments.

Description of key information

C16MES: 96hr LC50  = 1.5 mg/L (95% 1.13 - 1.99 mg/L) geom. mean of measured values

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.5 mg/L

Additional information

Additional information:

LC50 (96h) decreases with increasing carbon chain length

LC50 of the combined C14/C16 mix can be estimated from the LC50 of the separate components.

The combination toxicology cannot be used to determine one single key value for chemical safety assessment of the reaction mass - indeed, the proportion of both components that enter the environment (after the STP) is not deemed to be the same as the proportion of the 2 components before the STP