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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The 48h-EC50 exceeded an average exposure concentration of 0.31 mg/l, which was higher than the maximum water solubility (< 0.025 mg/l). 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.31 mg/L

Additional information

The acute toxicity to daphanie was conducted according to e OECD guideline No. 202, twenty daphnids per concentration (5 per replicate, 4 replicates) were exposed to a control and a WAF prepared at a loading rate of 100 mg/l. Additionally, 10 daphnids per concentration were exposed to dilutions containing 0.1, 1.0 and 10% of the WAF prepared at a loading rate of 100 mg/l. The test duration was 48 hours and samples for analytical confirmation of test concentrations were taken at the start and at the end of the test. The actual concentration in the sample taken from the WAF prepared at 100 mg/l at the start of the test was 0.76 mg/l. The measured concentration decreased to 0.13 mg/l at the end of the exposure period. Based on these results, the average exposure concentration was estimated to correspond to 0.31 mg/l.

Thus, 48h-EC50 is greater than 0.31 mg/L (measured concentration) under the test conditions, which was higher than the maximum water solubility (< 0.025 mg/l). Due to the very low solubility of Epikote P in water (< 0.025 mg/l), concentration levels that might be toxic for Daphnia magna could not be reached.