REAKTIV-ORANGE DYPR 1410

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01 February 1999 to 05 February 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L
- Sampling method: direct weighing
- Sampling: Daily analysis of the only test concentration plus a control.

Vehicle:

no

Details on test solutions:

Control: Synthetic fresh water in accordance with ISO

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Species: Zebra fish (Brachydanio rerio HAMILTON BUCHANAN)
Origin: Bio International B.V. (Netherlands)
Date of birth: 09.09.1998
Date supplied: 25.11.1998
Body length: 2.5 – 3.5 cm
Keeping conditions: SOP 2030-7700103-98 D
Mortality while kept: < 2%
Medical pre-treatment: none
Keeping water: Synthetic fresh water in accordance with ISO.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

No post observation period

Hardness:

13.5 mg CaCO3/L

Test temperature:

20.8-22.0

pH:

7.7-8.0

Dissolved oxygen:

8.8-9.6 mg/l

Salinity:

Not applicable.

Nominal and measured concentrations:

100 mg/l (nominal)

Details on test conditions:

Operating conditions: Static test in accordance with study method.
Photoperiod: 16 hours light / 8 hours dark
Test procedure: - test vessels (300 x 135 x 200 mm)
-51 test medium
-ventilated
-static
Method of administration: direct weighing (SOP 2030-6600401-96 D)
Test concentration/s: 100 [mg/1]
Solubilizer or other auxiliaries: none
Control: Synthetic fresh water in accordance with ISO.
Water for dilution: Synthetic fresh water in accordance with ISO.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 100 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Equates to >=79.6 mg/l of organic components.

Details on results:

The test result refers to a concentration of the test substance, which was calculated directly from analytically determined TOC values. According to the relevant product information, 1 mg/l TOC equals to 2.6 mg/l of the organic components of the test substance.
As these organic components correspond to 73%
of the molecular weight of the test substance 100 mg/l of the test substance equals to 28.1 mg/l organic C.
Thus, the above analytical values indicate a very good recovery rate.
Some test fish had a somewhat smaller size than the one recommended in the test guideline for the zebra fish. Deviating from the preliminary test, a mortality rate of 10% was detected in the main test at the limit test concentration of 100 mg/l. The validity criteria of the above test guideline permit a maximum mortality of 10% in the control.
Considering all test concentrations involved, temperature fluctuation was bigger than 1⁰C in the course of the study.

Results with reference substance (positive control):

Not applicable.

Sublethal observations / clinical signs:

Concentration of the test substance: 100 mg/l

Mortality

	2h	24h	48h	72h	96h
Absolute	0	0	1	0	0
Cumulative	0	0	1	1	1
Cumulative [%]	0	0	10	10	10

 

 

Concentration of the test substance: control

 

Mortality

	2h	24h	48h	72h	96h
Absolute	0	0	0	0	0
Cumulative	0	0	0	0	0
Cumulative [%]	0	0	0	0	0

 

Conclusions:

Exposure time: 96 h
arithmetic mean of analytical values
LC 0: >= 79.6 mg/l

Executive summary:

Study conducted to EU test guidance 92/66/EEC part C.1, in compliance with GLP.

Exposure time: 96 h

arithmetic mean of analytical values

LC 0:  >= 79.6 mg/l organic quotient.

LC0 overall: >=100 mg/l

The substance is not considered to be harmful to fish.

Description of key information

The LC50 was above 100 mg/L after 96 hours. The substance is not considered to be harmful to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L

Additional information

Study conducted to EU test guidance 92/66/EEC part C.1, in compliance with GLP.

Exposure time: 96 h

arithmetic mean of analytical values

LC0:  >= 79.6 mg/L organic quotient.

LC0 overall: >=100 mg/L

The substance is not considered to be harmful to fish.