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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
27-03-2018 to 29-03-2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Short term toxicity of test chemical to aquatic invertebrates was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Remarks:
acetone
Details on test solutions:
The stock solution 1.0 g/l was prepared by dissolving orange powder in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

ACCLIMATION - No data available
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Remarks on exposure duration:
± 1 hr
Test temperature:
20±1°C
pH:
sample at concentration 4.0 mg.l'1: pH = 7.8 changed to pH = 7.7 during the test,
control: = 7.8 changed to pH = 7.7 during the test
control + acetone: 7.9 changed to pH = 7.8 during the test
Dissolved oxygen:
higher than 7.6 mg/L at the end of test
Nominal and measured concentrations:
0, 0.25 , 0.50 , 1.00 , 2.00 , 4.00 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
3.91 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CI = 2.25 - 6.79 mg/l
Results with reference substance (positive control):
- Results with reference substance valid
- EC50:0.73 mg/L (24 hours)
Reported statistics and error estimates:
EC50 was calculated using non linear regression by the software Prism 4.0
Validity criteria fulfilled:
yes
Conclusions:
The median effective concentration (EC50) for the test substance on Daphnia magna was determined to be 3.19 mg/l on the basis of mobility inhibition effects in a 48 hour study.
Executive summary:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 1.0 g/l was prepared by dissolving orange powder in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water. 0, 0.25 , 0.50 , 1.00 , 2.00 , 4.00 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 3.19 mg/L on the basis of mobility inhibition effects in a 48 hour study. The water solubility of test substance was 0.11 mg/l. It was not possible to test higher sample concentrations due to low sample solubility. There was orange precipitate on the surface of the test medium at the concentrations higher than 0.25 mg/l. Result of 48h EC50 when all measured values are used: 48h EC50 = 3.91 mg/l, 95% CI = 2.25 - 6.79 mg/l.

Based on the above results, chemical can be consider to be toxic, however the obtained median effective concentration is very much higher than the solubility of the test chemical. Thus considering water solubility and toxicity values test chemical can be categorised into not classified category

Description of key information

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The stock solution 1.0 g/l was prepared by dissolving orange powder in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water. 0, 0.25 , 0.50 , 1.00 , 2.00 , 4.00 mg/lnominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 3.19 mg/L on the basis of mobility inhibition effects in a 48 hour study. The water solubility of test substance was 0.11 mg/l.It was not possible to test higher sample concentrations due to low sample solubility. There was orange precipitate on the surface of the test medium at the concentrations higher than 0.25 mg/l. Result of 48h EC50 when all measured values are used: 48h EC50 = 3.91 mg/l, 95% CI = 2.25 - 6.79 mg/l. Based on the above results, chemical can be consider to be toxic, however the obtained median effective concentration is very much higher than the solubility of the test chemical. Thus considering water solubility and toxicity values test chemical can be categorised into not classified category

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
3.19 mg/L

Additional information

Short termtoxicity of aquatic invertebrate:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The stock solution 1.0 g/l was prepared by dissolving orange powder in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water. 0, 0.25 , 0.50 , 1.00 , 2.00 , 4.00 mg/lnominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 3.19 mg/L on the basis of mobility inhibition effects in a 48 hour study. The water solubility of test substance was 0.11 mg/l.It was not possible to test higher sample concentrations due to low sample solubility. There was orange precipitate on the surface of the test medium at the concentrations higher than 0.25 mg/l. Result of 48h EC50 when all measured values are used: 48h EC50 = 3.91 mg/l, 95% CI = 2.25 - 6.79 mg/l. Based on the above results, chemical can be consider to be toxic, however the obtained median effective concentration is very much higher than the solubility of the test chemical. Thus considering water solubility and toxicity values test chemical can be categorised into not classified category