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Diss Factsheets

Administrative data

additional ecotoxicological information
Adequacy of study:
key study
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Data included and protocol for detection of AHTN in plants developed. Probable cross contamination from soil to leaves

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
other: Developed protocol for the determination of AHTN in plant materials.
Principles of method if other than guideline:
The analysis of AHTN is described in a seperated protocol that was included as an Annex in the study (Anhang B5-1)
GLP compliance:
not specified
Type of study / information:
The absorption of AHTN in lettuce and carrots in various AHTN spiked sewage sludge amended soils.

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
AHTN - Tonalid; no further specifications.

Results and discussion

Any other information on results incl. tables

The method has been validated. The accuracy in the measurement according to DIN 32 645 (1994), taking into account sample processing, is 1 µg/kg. The correctness of the procedure is based on the AHTN recovery rate, which is 98%.


The soil analysis for the soil/sewage mixture at the different locations has been measured over a period of 12 weeks (Table 3).

Greenhouse Outdoor
Week Borstel Friesland Latrop Control Borstel
0 37.2 29.3 31.8 n.d. 37.2
6 25.1 26.3 28.5 n.d. 28.4
8 26.8 31.0 30.7 n.d. 28.1
10 28.2 30.3 30.8 n.d. 30.4
12 30.6 36.4 35.4 n.d. 33.1
Average 29.6 30.6 31.4 n.d. 31.4

Table 3 Average AHTN content (mg/kg dw) in the test soils for the lettuce (n.d. non-detectable, detection limit 0.025 mg/kg dw).

Table 4 shows the AHTN content in the lettuce as function of time.

Greenhouse Outdoor
Week Borstel Friesland Latrop Control Borstel Control
6 584 384 215 47 854 28
8 183 218 239 30 667 2
10 226 207 191 101 957 67
12 107 198 190 21 476 13
Average 275 252 209 50 738 27

Table 4 Average AHTN concentration (µg/kg dw) in lettuce over the exposure period of 12 weeks.

The AHTN content in the soil remained constant and no degradation in the soil was observed. The average AHTN concentration was 245 µg/kg dw. No accumulation of AHTN was observed. Lettuce in the greenhouse showed a similar concentration around 0.2mg/kg dw after 8 weeks. The outdoor values are higher and this could possibly be due to rain splash from the AHTN spiked soil.


Analysis of the soil for the test of AHTN in root and leaves of carrots in the greenhoue can be found in the table below.

Week Friesland Latrop Control
0 29.3 31.8 n.d.
14 29.7 34.7 n.d.
16 35.6 35.9 n.d.
18 37.8 35.6 n.d.
Average 33.1 34.5 n.d.

Table 5 Average AHTN concentration (µg/kg dw) in lettuce over the exposure period of 12 weeks(n.d. non-detectable, detection limit 0.025 mg/kg dw).

The absorption of AHTN in carrot roots and leaves is shown in table 6

Root (mg/kg dw) Leaves (µg/kg dw)
Week Friesland Latrop Control Friesland Latrop Control
14 4.5 18.0 n.d. 189 1290 12
16 1.8 8.6 n.d. 75 1210 18
18 1.8 11.8 n.d. 82 212 13
Average 2.7 12.8 n.d. 115 902 15

Table 6 AHTN absorption in carrot roots (in mg/kg dw) and leaves (µg/kg dw)

In contrast to the lettuce, there is a dependency on the soil type.

Applicant's summary and conclusion