Registration Dossier

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: early study report, but basic information given

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1968
Report date:
1968

Materials and methods

Principles of method if other than guideline:
BASF-internal standard
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Trilon AS
IUPAC Name:
Trilon AS
Constituent 2
Chemical structure
Reference substance name:
Nitrilotriacetic acid
EC Number:
205-355-7
EC Name:
Nitrilotriacetic acid
Cas Number:
139-13-9
Molecular formula:
C6H9NO6
IUPAC Name:
2-[bis(carboxymethyl)amino]acetic acid
Details on test material:
H3.NTA ("TRILON AS"), solid

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of the same animal
Amount / concentration applied:
Test substance was applied as powder (1 x 50 mm3).
Duration of treatment / exposure:
24 hours
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
2

Results and discussion

In vivo

Results
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 8 d
Score:
3
Max. score:
3
Reversibility:
not reversible

Any other information on results incl. tables

Slight corneal opacity and severe edema after 1 h, erythema became severe while edema began to reverse after 24 h; slight corneal opacity persisted for 8 d while all other signs were reversible.

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information