Nitrilotriacetic acid

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: early study report, but basic information given

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Principles of method if other than guideline:

BASF-internal standard

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Trilon AS

IUPAC Name:

Trilon AS

Constituent 2

Reference substance name:

Nitrilotriacetic acid

EC Number:

205-355-7

EC Name:

Nitrilotriacetic acid

Cas Number:

139-13-9

Molecular formula:

C6H9NO6

IUPAC Name:

2-[bis(carboxymethyl)amino]acetic acid

Details on test material:

H3.NTA ("TRILON AS"), solid

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eye of the same animal

Amount / concentration applied:

Test substance was applied as powder (1 x 50 mm3).

Duration of treatment / exposure:

24 hours

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Results and discussion

In vivo

Results

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 8 d

Score:

3

Max. score:

3

Reversibility:

not reversible

Any other information on results incl. tables

Slight corneal opacity and severe edema after 1 h, erythema became severe while edema began to reverse after 24 h; slight corneal opacity persisted for 8 d while all other signs were reversible.

Applicant's summary and conclusion

Interpretation of results:

slightly irritating

Remarks:

Migrated information