Butanedioic acid, sulfo-, monoesters with lanolin alcs., disodium salts

Short-term toxicity to aquatic invertebrates

Description of key information

IC50  > 100 mg/L of active substance

NOEC = 100 mg/L of active substance

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The acute toxicity of the test item Sulfosuccinate of lanolin Alcohol to Daphnia magna was determined in a 48‑hour full static test according to the OECD Guideline for Testing of Chemicals, No. 202 (2004)

For this purpose, juvenile daphnids (< 24 hours old at initiation of the test) were exposed to an aqueous test medium containing the active ingredient at five different concentrations, namely 4.3, 9.4, 20.7, 45.5 and 100.0 mg/L, corresponding to 14.2, 31.3, 68.9, 151.5 and 333.3 mg/L as test item.

The exposed organisms were checked for immobilization 24 and 48 hours after test initiation.

The pH of the test media after preparation was in the range 7.27 – 7.51 for test item solutions and 7.29 for negative control.

The incubation temperature was in the range 18.7 – 21.2 °C.

Light intensity during the 48 hours of test period was in the range 780 - 797 Lux.

At the end of test period, in the negative control 0 % of immobilization was observed and no daphnid showed signs of disease or stress (e.g. to be trapped on the test water surface). These values comply with the validity criteria of the test, that provide a maximum immobilization or a maximum number of daphnids showing disease or stress signs of 10 % in the negative control medium at the end of the test.

The obtained experimental results allowed to calculate the IC₀, IC₁₀₀ the IC₅₀values and the NOEC at 24 and 48 hours.

The IC50 was defined > 100 mg/L of active substance (> 333.3 mg/L of test item), the NOEC 100 mg/L of active substance ( 333.3 mg/L nominal concentration of the test item).