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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008-06-25 to 2008-06-27
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
The analytical monitoring was conducted at three test concentrations (1.5, 16.5 and 80 mg/l) at the start (0h) and at the end of the test (48h). After
48h, 2 ml-samples were taken both from additional test vessels (without Daphnia) and from the test vessels with Daphnia to check the influence of the test organisms.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: a stock solution of 80 mg/l was prepared with dilution water; seven test solutions with concentrations ranging from 0.7 to 80 mg/l were
were prepared from this stock solution
- Controls: dilution water
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: from a clone breeding at the German Federal Environmental Agency (UBA), Berlin
- Age at study initiation: 4-23 h
- Method of breeding:
- Feeding during test: no

ACCLIMATION
- Acclimation period: 4 h at 20 +/- 2 °C in the dark
- Acclimation conditions: same as test

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20-21 °C
pH:
control: 7.8 - 8.5
0.7 mg/l: 7.9 - 7.9
1.5 mg/l: 7.9 - 8.2
3.4 mg/l: 8.0 to 8.2
7.5 mg/l: 8.0 ot 8.1
16.5 mg/L: 8.1 to 8.3
36.4 mg/L: 8.0 to 8.7
80 mg/L: 9.2 to 8.2
Dissolved oxygen:
control: 7.6 to 8.0 mg/l
0.7 mg/l: 7.6 to 8.0 mg/l
1.5 mg/l: 7.6 to 7.9 mg/l
3.4 mg/l: 7.7 to 8.1 mg/l
7.5 mg/l: 7.7 to 8.0 mg/l
16.5 mg/L: 7.7 to 8.0 mg/l
36.4 mg/L: 7.6 to 7.9 mg/l
80 mg/L: 7.7 to 8.0 mg/l
Nominal and measured concentrations:
A: 0.7, B: 1.5, C: 3.4 ,D: 7.5, E: 16.5, F: 36.4 and G: 80.0 mg/l (nominal)
B: 1.46, E: 16.1, G: 78.8 mg/l (measured, 0 h)
B: 1.47, E: 16.3, G: 79.4 mg/l (measured, 48 h, with animals)
B: 1.5, E: 16.6, G: 85.7 mg/l (measured, 48 h, without animals)
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml-beakers, filled with 20 ml test medium each
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- ISO test water

EFFECT PARAMETERS MEASURED:
- immobility at 0 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study: yes (non-GLP
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
7.71 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 6.23-9.55
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
20.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 14.98-26.96
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
3.88 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 2.76-5.45
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
7.14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 4.20-12.11
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
3.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: no
- Mortality of control: no
- Other adverse effects control: no

At the higher test concentrations, more immobilised Daphnia were observed after 24h than after 48h. The results indicate that apparently the Daphnia were temporarily intoxicated at 36.4, 16.5 and 7.5 mg/l. After 48 h, recovery took place, explaining the higher 48h-EC50.
Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 1.12 mg/l (CL, 95 %: 1.02-1.24 mg/l)
Reported statistics and error estimates:
EC10-, EC20- and EC50-values (including confidence limits) as well as the concentrations demonstrating no effect (NOEC) were calculated for both
assessments (24 and 48h) using the statistical software ToxRat (ToxRat solutions GmbH, Alsdorf)

The recovery rates [%] for the test item in the test solutions B (1.5 mg/l), E (16.5 mg/l) and G (80 mg/l) were as follows:

At the start of the test (0h):

B: 97.3%, E: 97.6%, G: 98.5%

At the end of the test (48h):

B: 98.0%, E: 98.8%, G: 99.3% (with animals)

B: 100.0%, E: 100.6%, G: 107.1% (wihtout animals)

Validity criteria fulfilled:
yes
Conclusions:
The EC50 (48h) based on nominal concentrations was determined as 20.1 mg/L indicating that the test substance may be harmful to aquatic invertebrates.
Executive summary:

The 48-h acute toxicity of decamethylenediamine to Daphnia magna was studied under static conditions according to OECD TGD 201. Daphnids were exposed to the test item at nominal concentrations of 0.7, 1.5, 3.4, 7.5, 16.5, 36.4 and 80.0 mg/l for 48 h. Immobilisation was observed.

The 48h-EC50 was 20.10 mg/L.

This study was assessed as "reliable without restrictions".

Description of key information

The 48-h acute toxicity of decamethylenediamine to Daphnia magna was studied under static conditions according to OECD TGD 201. Daphnids were exposed to the test item at nominal concentrations of 0.7, 1.5, 3.4, 7.5, 16.5, 36.4 and 80.0 mg/l for 48 h. Immobilisation was observed. The 48h-EC50 was 20.10 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
20.1 mg/L

Additional information