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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
study only continued for 24 hours
GLP compliance:
not specified
Analytical monitoring:
yes
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Total exposure duration:
24 h
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
ca. 2.5 mg/L
Nominal / measured:
nominal
Remarks on result:
other: Technical product, undiluted
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
ca. 4 mg/L
Nominal / measured:
nominal
Remarks on result:
other: Technical product, diluted in Acetone
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
ca. 1.5 mg/L
Nominal / measured:
nominal
Remarks on result:
other: Sublimed and uncrystallised products, diluted in acetone
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
ca. 0.2 mg/L
Nominal / measured:
nominal
Remarks on result:
other: technical, diluted in acetone
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
ca. 0.5 mg/L
Nominal / measured:
nominal
Remarks on result:
other: sublimed and uncrystalised products, diluted in acetone
Validity criteria fulfilled:
yes
Conclusions:
3 forms of ferrocene were tested in a study performed that was similar to OECD Guideline 202. Between the 3 different forms, EC50 values showed no hugely significant differences. Technical grade product diluted in Acetone gave EC50 24hr = 4 mg/l, sublimed and uncrystallised products both diluted in acetone gave EC50 24hr = 1.5 mg/l. They show however, that in acute tests daphnia magna are somewhat more sensitive to ferrocene than fish. For a CSA value as this study was only performed for 24 hours, the EC50 values have been decreased by 50% to allow for 48 hour exposure. Therefore technical grade product diluted in Acetone has an EC50 48hr = 2 mg/l with sublimed and uncrystallised products both diluted in acetone, having EC50 48 hr = 0.75 mg/l. Value used in CSA is EC50 (48hr) = 1.17 mg/l

Description of key information

In a study performed to guidelines similar to OECD 202, 3 forms of ferrocene were tested.


Technical grade product diluted in Acetone has an EC50 (48 hr) = 2 mg/l and the sublimed and uncrystallised products both diluted in acetone have EC50 (48 hr) = 0.75 mg/l.


Average EC50 for the 3 forms used in CSA is EC50 (48hr) = 1.17 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
1.17 mg/L

Additional information

3 forms of ferrocene were tested in a study performed that was similar to OECD Guideline 202. Between the 3 different forms, EC50 values showed no hugely significant differences. Technical grade product diluted in Acetone gave EC50 24hr = 4 mg/l, sublimed and uncrystallised products both diluted in acetone gave EC50 24hr = 1.5 mg/l. They show however, that in acute tests daphnia magna are somewhat more sensitive to ferrocene than fish. As this study was only performed for 24 hours for a CSA value , the EC50 values have been decreased by 50% to allow for 48 hour exposure. Therefore technical grade product diluted in Acetone has an EC50 48hr = 2 mg/l with sublimed and uncrystallised products both diluted in acetone, having EC50 48 hr = 0.75mg/l.

Value used in CSA is EC50 (48hr) = 1.17 mg/l