3-aminopropan-1-ol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1958

Report date:

1958

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The test substance in the amount indicated was applied to the conjunctival sac of rabbits. The non-treated adjacent eye served as control. The animals were observed several times on the treatment day and 8 days afterwards, and findings were recorded on working days. The eyes were not washed out after 24 hours after substance application. Findings were graded as described in OECD test guideline 405.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-aminopropan-1-ol

EC Number:

205-864-4

Cas Number:

156-87-6

Molecular formula:

C3H9NO

IUPAC Name:

3-aminopropan-1-ol

Test material form:

liquid

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

no data

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: concurrent NaCl

Amount / concentration applied:

one drop (ca. 0.05 mL)

Duration of treatment / exposure:

single application

Observation period (in vivo):

24 days

Number of animals or in vitro replicates:

4

Details on study design:

SCORING SYSTEM: no scores used in the raw data

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

24 h

Reversibility:

not reversible

Remarks on result:

other: no scores used in raw data

Results 2

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

24 h

Reversibility:

not reversible

Remarks on result:

other: no scores used in raw data

Results 3

Irritation parameter:

cornea opacity score

Basis:

animal #3

Time point:

24 h

Reversibility:

not reversible

Remarks on result:

other: no scores used in raw data

Results 4

Irritation parameter:

cornea opacity score

Basis:

animal #4

Time point:

24 h

Reversibility:

not reversible

Remarks on result:

other: no scores used in raw data

Results 5

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

48 h

Remarks on result:

not measured/tested

Results 6

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

48 h

Remarks on result:

not measured/tested

Results 7

Irritation parameter:

cornea opacity score

Basis:

animal #3

Time point:

48 h

Remarks on result:

not measured/tested

Results 8

Irritation parameter:

cornea opacity score

Basis:

animal #4

Time point:

48 h

Remarks on result:

not measured/tested

Results 9

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

72 h

Remarks on result:

not measured/tested

Results 10

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

72 h

Remarks on result:

not measured/tested

Results 11

Irritation parameter:

cornea opacity score

Basis:

animal #3

Time point:

72 h

Remarks on result:

not measured/tested

Results 12

Irritation parameter:

cornea opacity score

Basis:

animal #4

Time point:

72 h

Remarks on result:

not measured/tested

Results 13

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Remarks on result:

not measured/tested

Results 14

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

24/48/72 h

Remarks on result:

not measured/tested

Results 15

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Remarks on result:

not measured/tested

Irritant / corrosive response data:

One drop of the undiluted test substance applied to the conjunctival sac of the eye produced very strong reddening within 10 min combined with enhanced secretion, corneal opacity, and slight chemical burning of the mucosa. The signs of mucosal irritation/corrosion were visible for at least 3 days. On day 3 ciliar injections and mucosal bleedings were observed combined with smeary secretion, slight chemosis and diffuse corneal opacity. After 6 days clear signs of irreversible corneal damage and iritis were observable including scar formation and cloudy corneal opacity. At day 8 slight reddening, slight mucosal bleedings, smeary secretion and cloudy corneal opacity were still visible.
At the end of the study period (day 24) irreversible signs of corneal damage were observed as well as ciliar injections in the lower half of the eyeball in two animals. Two animals were euthanized on day 6 of the study due to massive chemical burnings of the eye including black-brown necroses, strong bleedings/bloody secretions and foggy corneal opacity.

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye) based on GHS criteria

Conclusions:

3-Aminopropan-1-ol causes severe eye damage.
At the end of the study period (day 24) irreversible signs of corneal damage were observed as well as ciliar injections in the lower half of the eyeball in two animals. Two animals were euthanized on day 6 of the study due to massive chemical burnings of the eye including black-brown necroses, strong bleedings/bloody secretions and foggy corneal opacity.