Reaction products of fatty acid chlorides, C8-12 (even numbered) with glycine and sodium hydroxide

Toxicity to microorganisms

Description of key information

The toxicity to microorganisms of the registration substance Hostapon SLG was evaluated based on the read-across approach using the toxicity to microorganisms data of Hostapon SG.

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item Hostapon SG (batch number:ESD0006364) from 2012-01-10 to 2012-02-07, with the experimental phase on 2012-02-07 atDr.U.Noack-Laboratorien, D-31157Sarstedt.Test system was activated sludge of the municipal treatment plant of D-31137 Hildesheim. The test was carried out under static conditions with the nominal active ingredient concentrations 100 - 180 - 320 - 580 - 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from -33 % to 62 %.

In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC₅₀-value for the reference item was 129 mg/L.

Table 1:   EC-Values and NOEC of Hostapon SG (a.i.)

	Effect values (nominal a.i.)	Confidence interval
	[mg/L]	P = 95 [%] [mg/L]
NOEC	320	–
EC10	372	347 – 398
EC20	452	422 – 486
EC50	791	733 – 847
EC80	> 1000	n.d.

Key value for chemical safety assessment

EC50 for microorganisms:

791 mg/L