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EC number: 239-415-9 | CAS number: 15396-00-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-08-26 to 2003-01-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0(Control) and 100 mg/l
- Sampling method: Duplicate samples of test media were taken at the start and end of the test.
- Sample storage conditions before analysis: samples were analysed immediately after collection. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Three, four litre volumes of test media with a nominal concentration of 100 mg/l were prepared by intense stirring for 24 hours. The three volumes were then aggregated to produce the final test medium.
- Controls: Dilution water - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Forellenzuchtbetrieb Tautenhahn, 98646 Trostadt, Germany
- Length at study initiation (length definition, mean, range and SD): 5.13 +/-0.39 cm
- Weight at study initiation (mean and range, SD): 1.26 +/-0.23 g
ACCLIMATION
- Acclimation period: 4 weeks
- Acclimation conditions (same as test or not): yes
- Type and amount of food: commercial fish food
- Health during acclimation (any mortality observed): no mortalities observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 250 mg/l as CaCO3
- Test temperature:
- 15-17ºC
- pH:
- 7.7-7.9
- Dissolved oxygen:
- =8.7 mg/l
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0(Control) and 100 mg/l
Measured concentrations were 88% of nominal at the start of the test and 76% at the end of the test. The mean measured concentration was therefore 82 mg/L.
The test results are reported and interpreted with reference to nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 20 litre capacity with 12 litre of test medium
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.74 g/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater prepared by adding analytical grade salts to deionised water.
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: 320-420 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality after 2, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Range finding study
- Test concentrations: not reported
- Results used to determine the conditions for the definitive study: not reported - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: none
- Mortality of control: 0 - Reported statistics and error estimates:
- There were no toxic effects observed in the test and therefore no statistical tests were required.
- Sublethal observations / clinical signs:
No toxic effects were observed following 96-hour exposure to a nominal concentration of 100 mg/l.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96-hour LC50 value of >100 mg/L and NOEC of =100 mg/L have been determined for the effects of the test substance on mortality of Oncorhynchus mykiss based on nominal concentration of the substance. The test substance is susceptible to hydrolysis and it is likely that the test organisms were primarily exposed to the hydrolysis products of the substance.
Reference
Description of key information
Short-term toxicity to fish: 96 hr LC50 >100 mg/L (nominal) (limit test) (OECD 203), or >67 mg/L when expressed in terms of concentration of the silanol hydrolysis product 3-aminopropylsilanetriol.
Key value for chemical safety assessment
Additional information
A 96-hour LC50 value of >100 mg/L (highest concentration tested) has been determined for the effects of the registration substance on mortality of Oncorhynchus mykiss based on nominal concentrations of the substance (IBACON, 2003a).
The isocyanate group is very rapidly hydrolysed to the corresponding amine (half-life of <5 minutes or less), and further hydrolysis of the alkoxy groups proceeds at a slower rate (half-life of 2.6 h at pH 7 and 20-25°C, estimated) to 3-aminopropylsilanetriol and methanol, therefore, it is likely that the test organisms were primarily exposed to the hydrolysis products of the substance during the static test.
The results may be expressed in terms of concentration of the hydrolysis product, 3-aminopropylsilanetriol, by applying a molecular weight correction: (MW of silanol = 137.21 / MW of parent = 205.29) * >100 mg/L = >67 mg/L as 3-aminopropylsilanetriol.
Reliable supporting data are read across from an appropriate structural analogue, 3-aminopropyl(triethoxy)silane (CAS 919-30-2), which is a close structural analogue of 3-(trimethoxysilyl)propylamine (CAS 13822-56-5), the amine which is formed very rapidly when the registered substance comes into contact with water/moisture. 3-Aminopropyl(triethoxy)silane (CAS 919-30-2) also reacts in water to the same final hydrolysis product as the registration substance, 3-aminopropylsilanetriol (half-life of 8.5 hours at pH 7 and 25 °C).
A 96-hour LC50 of >934 mg/L (measured DOC concentration) (highest concentration tested) has been determined for the effects of 3-aminopropyl(triethoxy)silane (CAS 919-30-2) on mortality of Brachydanio rerio (tested as Danio rerio), in accordance with OECD Test Guideline 203 (Hüls, 1994).
The test substance is susceptible to hydrolysis and it is likely that the test organisms were predominantly exposed to the hydrolysis products of the substance.
The results may be expressed in terms of concentration of the hydrolysis product, 3-aminopropylsilanetriol, by applying a molecular weight correction: (MW of silanol = 137.21 / MW of parent = 221.37) * >934 mg/L = >579 mg/L. This value is read across to 3-(trimethoxysilyl)propyl isocyanate (CAS 15396-00-6) without further correction.
In addition, short-term toxicity to fish data have been read-across from 3-(trimethoxysilyl)propylamine (CAS 13822-56-5). This substance is the same as the amine which is formed very rapidly when the registered substance comes into contact with water/moisture. Both substances then hydrolyse at the same rate to form the hydrolysis products 3-aminopropylsilanetriol and methanol (half-life of 2.6 h at pH 7 and 20-25°C, estimated). However, the result is of non-assignable reliability, taken from a secondary data source. An LC50 value of 1264 mg/L was reported (Chandra, 1997).
Condensation reactions leading to insoluble oligomers/polymers can be a problem for ecotoxicity testing with substances generating silanols. This has been considered for this substance and is not considered to have been an issue at the test media stock concentration up 1000 mg/L in the key studies (PFA, 2016am).
Refer to the IUCLID Section 6 endpoint summary (Section 7.0 of the CSR) for further discussion of the approach to chemical safety assessment for this registration substance, and for justification for read-across used.
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