Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 215-481-4 | CAS number: 1327-53-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- 48-h acute toxicity test with Daphnia magna
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Purity: reagent grade
NaAsO2 - Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken at 0, 48, and 96 hr for the daphnid acute toxicity tests.
Five mL samples of exposure water and 50mL of control tank water were collected and diluted to near 100mL with deionized water. Before final volume adjustment, samples were acidified(1%HNO3,v/v) and fortified with 0.6% hydrogenperoxide (H202, v/v) and 0.1% nickel nitrate hexahydrate (Ni(NO3)z-6H20, w/v). Sample digestion was omitted. - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnids were also obtained from Environmental Research Laboratory-Duluth (ERL-D), Duluth, MN stock culture.
Life stage: < 24h neonates
They were rearedin 25°C Lake Superior water and fed a mixture of ground trout pellets and yeast(ASTM 1980).
The food mixture was prepared by homogenizing 60 g of fish food pelletswith 1.5 g of bread-makers yeast in water. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 46.3-49.9 mg/L CaCO3
- Test temperature:
- 15.6 °C
- pH:
- 7.2-8.1
- Dissolved oxygen:
- 79.6-99.3%
- Nominal and measured concentrations:
- 0.815, 1.08, 1.86, 2.44, 4.19 and 6.46 mg As/L (<0.002 mg As/L in control)
Test concentration were analytically confirmed. - Details on test conditions:
- An acute toxicity test with As and daphnids were completed at the UW-Superior laboratory without food added.
The exposure chambers were 250mL beakers containing 200 mL of the toxicant solution.
Six exposure concentrations and controls were prepared in duplicate for the unfed and fed tests.
The chambers were placed in a temperature controlled water bath and five daphnids,<24 hr old, were placed in each chamber. - Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element (dissolved fraction)
- Remarks:
- As
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% confidence interval: 1.17 - 1.94 mg As/L
- Reported statistics and error estimates:
- Trimmed Spearman-Karbemr method
- Conclusions:
- The short-term 48 hours LC50 value reported for freshwater invertabrate is 1.500 mg As/L for the mortality of Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM 1980. Standard practice for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians
- Principles of method if other than guideline:
- short-term toxicity test to Opossum shrimp
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Test organisms (species):
- Americamysis bahia (previous name: Mysidopsis bahia)
- Test type:
- flow-through
- Water media type:
- saltwater
- Total exposure duration:
- 96 h
- Test temperature:
- 20-25 °C
- pH:
- 7.8-8.2
- Salinity:
- 30 ppt
- Nominal and measured concentrations:
- Test concentrations were analytically confirmed.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.74 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element (dissolved fraction)
- Remarks:
- As
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% confidence limits: 1.39 - 2.26 mg As/L
- Conclusions:
- The 96-h LC50 for survival of the marine invertebrate Americamysis bahia is 1.74 mg As/L.
Referenceopen allclose all
Description of key information
Six reliable data points were retained for acute toxicity of As to freshwater invertebrates, covering four different invertebrates (i.e. the crustaceans C. dubia, D. magna, D. pulex and G. pseudolimnaeus). L(E)C50 values range between 1448 and 11900 µg As/L. The lowest value of 1448 µg As/L was observed for C. dubia, whereas the geometric mean value of the three data points for C. dubia was 1579 µg As/L. The lowest species mean 48 -h LC50 value for D. magna of 1500 µg As/L is put forward for classification purposes.
In addition, one reliable datapoint was identified for a standard test with the marine opossum shrimp Americamysis bahia with a reported 96-h LC50value of 1740 µg As/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.5 mg/L
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 1.74 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
