Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

OECD 471, Mutagenicity on Bacteria: All the available studies (BSA, TSA, CSA, HBSA) showed no toxicity and no mutagenic effects at any dose level with any tester strain, in the absence and presence of S9 metabolic activation. Since there was no evidence of mutagenicity observed for any of the compounds with and without metabolic activation, it could be concluded that all the ASA category members does not induce reverse mutation in Salmonella Typhimurium and Escherichia coli. The substance for which the test is missing (XSA) is assessed with the available data on the related salt (SXS).


OECD 476: The tested ASA (BSA, HBSA) did not increase the frequencies of gene mutations in mammalian cells. The substances for which the test is missing are assessed with the available data considering that the difference is only in the type of salt which is not considered to influence the behaviour of the substances. The other ASA substances for which the test is missing (TSA, CSA and XSA) are assessed with the available data on the related salts (respectively STS, SCS and SXS).


OECD 473: The tested ASA (BSA, TSA, HBSA) did not show any mutagenic effects on in vitro chromosome aberration test.
The substances for which the test is missing (CSA and XSA) are assessed with the available data on the related salts (respectively in vivo OECD 474 on SCS and in vitro OECD 473 on SXS).

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (negative)

Genetic toxicity in vivo

Description of key information

OECD 474: There are no in vivo data on ASA but they are available in vivo studies for Hydrotropes. SCS and CaXS don't show any mutagenic effects and this results can be applied akso to the related acid.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (negative)

Additional information

Justification for classification or non-classification

The ASA Category comprises the following 5 aromatic sulphonic acids:


TSA, Toluene-4-sulphonic acid (EC 203-180-0, CAS 104-15-4)


XSA, (Xylenes and 4-ethylbenzene) sulphonic acid (EC 701-247-3, CAS -) Former EC 246-839-8


CSA, p-cumene sulphonic acid (EC 240-210-1, CAS 16066-35-6)


BSA, Benzene sulphonic acid (EC 202-638-7, CAS 98-11-3)


HBSA, 4-hydroxybenzensulphonic acid (EC No. 202-691-6, CAS No. 98-67-9)


There are available mutagenic data on all the ASA substances and all the results showed the absence of mutagenic effect. Since no positive results were seen in any of the studies, the chemicals in the ASA category is considered not to have a genotoxic or mutagen potential. The density of the mutagenic data is high enough to consider the hydrotropes as a category and the available information support a regular pattern of same absence of and type of effects with regard to mutagenicity. The substances for which the test is missing are assessed with the available data on the other ASA members and related salts members of the Hydrotropes Category.