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Toxicity to soil macroorganisms except arthropods

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Description of key information

The toxicity to terrestrial macroorganisms was determined in a study according to OECD guideline No 222 and in compliance with GLP criteria (BASF, 2016). In this study, groups of 10 earthworms (E. fetida) were exposed to nominal test substance concentrations of 0 (control), 62.5, 125, 250, 500 and 1000 mg/kg soil d.w. Artificila soil was used consisting of 73.7 % quartz sand, 21.0 % kaolin clay, 5.1 % sphagnum peat and 0.2 % CaCO3. No test substance analysis was performed. Number and biomass [mg] of the adult earthworms was determined at the start of exposure and after 28 days of the test. Reproduction, as mean number of juveniles per test vessel, was determined after 56 days exposure. The results in this study are consistent with all validity criteria and the test is valid according to the test guideline of this study.

No significant effects on mortality, biomass or reproduction were noted at any of the tested concentrations. Based on these findings the NOEC for all these effect partameters was determined at 1000 mg/kg soil d.w.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
1 000 mg/kg soil dw

Additional information