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Diss Factsheets
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EC number: 203-632-7 | CAS number: 108-95-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The lowest EC50-value of 21 mg/L for microorganisms relevant for waste water treatment plants was acquired in a 24 h nitrification test with a nitrifying enrichment culture obtained from the mixed liquor of an activated sludge plant treating meat-packing, rendering, and hide-curing waste-water.
The toxicity of phenol for Nitrosomonas sp. was tested. The seed bacteria for the nitrifiying enrichment culture were obtained from the mixed liquor of an acitivated sludge plant treating meat-packing, rendering, and hide-curing waste-water. The reactor vessel was a 20 L glass bottle. Diffused aeration provided complete mixing and aeration. The culture was fed two times per day apporximately 1000 mg/L ammonia-nitrogen in an inorganic nutrient solution buffered by sodium bicarbonate. The inhibition of ammonium consumption was used as the criterion for toxic inhibition of Nitrosomonas. Sealed serum bottles (125 mL) were prepared with ammoniua feed and 20 mL of surcharged oxygen. Ammonia was measured at the end of the assay period using an ammonia selective elctrode. Nitrite was checked to ensure that only toxicity to Nitrosomonas and not toxicity to Nitrobacterer was controlling the rate of metabolism activity. A range of toxicant concentrations were tested. Toxicants were tested in at least two experiments. An average IC50 of 21 mg/L was calculated.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 21 mg/L
Additional information
The data on effects of phenol on microorganisms is presented in EU RAR (2006) in Section 3.2.1 (Aquatic compartment (incl. Sediment), at page 42ff). It is stated that as a worst case assumption the EC50 of 21 mg/L (Blum et al., 1991) was used in the risk characterisation for waste water treatment plants.
Results of tests conducted with manifold microorganisms (e.g. waste water sludge, methanogens, activated sludge, several single species) are available. The test methods also vary from short-term tests over 5 min to cell multiplication inhibition test over 48 h. The nitrification (EC50 21 mg/L, Blum et al., 1991) test was selected as key study due to following reasons:
- mixed culture of natural source representative for waste water treatment plants
- sensitive endpoint
- long-term test
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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