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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

It can be concluded that the iron ion is the most toxic component of iron trinitrate.  Therefore, the mean 96 hour LC50 of 0.88 mg Fe/L obtained from three studies with Salvelinus fontinalis over a range of pH values will be as the key endpoint. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.88 mg/L

Additional information

Acute studies with fresh water fish have been conducted with iron sulphate and sodium nitrate.

The iron sulphate studies were conducted with Salvelinus fontinalis and provided 96 hour LC50endpoints of 0.41, 0.48 and 1.75 mg Fe/L (mean: 0.88 mg/L) at pHs of 5.5, 6.0 and 7.0 respectively.  These concentrations significantly exceed the true solubility of iron under the test conditions and it is therefore likely that physical effects, as well as pH effects, contributed to the observed response.

The toxicity of nitrate (NO3) to Pimephales promelas was assessed in three 96-hour acute toxicity tests. The lowest 96-hour LC50 from three acute tests was 1010 mg/L NO3-N. This equates to an LC50 for nitrate of 4472 mg/L NO3.

It can be concluded that the iron cation is the most toxic component of iron trinitrate and therefore the mean LC50of 0.88 mg Fe/L obtained across the pH range will be used for inter-species comparisons.