disodium 4-amino-6-((4-((4-(2,4-diaminophenyl)azo)phenylsulfamoyl)phenyl)azo)-5-hydroxy-3-((4-nitrophenyl)azo)naphthalene-2,7-disulfonate

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 11, 2009-November 21, 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

EU Method A.8 (Partition Coefficient)

GLP compliance:

no

Type of method:

shake-flask method to: flask method

Partition coefficient type:

octanol-water

Analytical method:

other: spectrophotometry UV-Vis

Type:

log Pow

Partition coefficient:

ca. -1.73

pH:

ca. 8.64

Remarks on result:

other: T n.a.

Table No.3 Results of determination of partition coefficient

Ratio o/w	Water phase		n-octanol phase		Pow	log Pow	Average log Pow
	c (g/L)	Average (g/L)	c (g/L)	Average (g/L)
50/50	0.04516	0.04786	0.00091	0.00090	0.0202	-1.6937	-1.7250
50/50	0.05057		0.00089		0.0175	-1.7563
60/30	0.04837	0.04871	0.00075	0.00068	0.0154	-1.8115	-1.8581
60/30	0.04904		0.00061		0.0125	-1.9047
30/60	0.05025	0.05045	0.00127	0.00124	0.0252	-1.5989	-1.6104
30/60	0.05065		0.00121		0.0239	-1.6220

    

Average of P_owwas 0.0191; standard deviation of P_owwas 0.0049.

Average of log P_owwas –1.73, standard deviation of log P_owwas 0.12

The partition coefficient of test substance, Acid Black 210, is: log P_ow =–1.73.

 

Conclusions:

The partition coefficient of the tested substance Acid Black 210, is logPow= -1.73

Executive summary:

The partition coefficient n-octanol/wtaer of Acid Black 210 was assessed following EU A8, by shake flask method and spectrophotometric determination of the concentrations in water and n-octanol.

The log Pow is equal to -1.73

Description of key information

-1.73

Key value for chemical safety assessment

Log Kow (Log Pow):

-1.73

at the temperature of:

25 °C

Additional information

A study was performed on Acid Black 210, following EU A8 by shake flask method (Stahl, 1996). The summary available is lacking of many information and therefore the test is used as supporting test.  The log Kow at 25°C is equal to -3.1.

Another study, used as key study, (Michal Bartos, 2011) was performed on Acid Black 210 according to EU method A.8.  At pH 8.6 the log Kow is equal to -1.73.