Ammonium acetate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

results for 48 hours

GLP compliance:

not specified

Analytical monitoring:

no

Details on sampling:

- Concentrations: range of total ammonia concentrations tested (mg[NH4+ and NH3]/dm3): 45-95.

Vehicle:

no

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Common carp
- Weight at study initiation (mean and range, SD): 42.5 ± 20.0 g

ACCLIMATION
- Acclimation period: 10-14 days
- Type and amount of food: no food.

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

Total hardness (mval). 3.20-3.34

Test temperature:

16.0 (14.0-17.6) ºC

pH:

7.6 (7.3-8.1)

Dissolved oxygen:

8.5 (7.2-9.6) mg/dm3

Nominal and measured concentrations:

- Concentrations: range of total ammonia concentrations tested (mg[NH+4 and NH3]/dm3): 45-95.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 245 dm3
- Aeration: steady aireation
- Renewal rate of test solution (frequency/flow rate): every 24 hours
- No. of organisms per vessel: 6

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

308 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

The acute toxicity of Ammonium acetate (48 hours) LC50 was determined to be 308 mg/L (72 mg [NH4 + and NH3]/L with 95% confidence interval: 55 -94 mg [NH4 + and NH3]/L).

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of Ammonium acetate (48 hours) LC50 was determined to be 308 mg/L (72 mg [NH4 + and NH3]/L with 95% confidence interval: 55 -94 mg [NH4 + and NH3]/L).

Executive summary:

Acute toxicity of Ammonium acetate to Common carp was determined. Experiments were carried out under static conditions; the tested substance was replaced after 24h.

The acute toxicity of Ammonium acetate (48 hours) LC50 was determined to be 308 mg/L (72 mg [NH4 + and NH3]/L with 95% confidence interval: 55 -94 mg [NH4 + and NH3]/L).

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: No data on test method nor on GLP.

Principles of method if other than guideline:

No data provided on the method.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Gambusia affinis

Details on test organisms:

TEST ORGANISM
- Common name: Mosquitofish

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

24 h

Nominal and measured concentrations:

No data

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

other: TLm

Effect conc.:

238 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (TLm is the median tolerance limit)

Sublethal observations / clinical signs:

The (24 h) TLm for Mosquito fish was 238 mg/L.

Validity criteria fulfilled:

not applicable

Conclusions:

The (24 h) TLm for Mosquito fish was 238 mg/L.

Executive summary:

The (24 h) TLm for Mosquito fish was 238 mg/L. Conditions of bioassay were not specified.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: No data provided on the method.

Principles of method if other than guideline:

No data provided on the method.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Gambusia affinis

Details on test organisms:

TEST ORGANISM
- Common name: Mosquito fish

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

96 h

Nominal and measured concentrations:

No data

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

238 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

238 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

The (24 -96 h) LC50 for Mosquito fish was 238 mg/L.

Validity criteria fulfilled:

not applicable

Conclusions:

The (24 -96 h) LC50 for Mosquito fish was 238 mg/L.

Executive summary:

The (24 -96 h) LC50 for Mosquito fish was 238 mg/L. Conditions of bioassay were not specified.

Description of key information

Short-term toxicity to fish:  Experimental data on Ammonium Acetate, LC50  is 308 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

308 mg/L

Additional information

Experimentals results on Ammonium Acetate:

In the publication reported by Dabrowska and Sikora (1986), a scientifically defensible approach was used to conduct the study, similar to OECD 203 guideline. The acute toxicity of Ammonium acetate (48 hours) LC50 was determined to be 308 mg/L (72 mg [NH4+and NH3]/L with 95% confidence interval: 55 -94 mg [NH4+ and NH3]/L).

In secondary sources, a (24 -96 h) TLm for Mosquito fish was reported as 238 mg/L. The TLm is the median tolerance limit. Conditions of bioassay were not specified.