Calcium 3-hydroxy-4-[(4-methyl-2-sulphonatophenyl)azo]-2-naphthoate

Sediment toxicity

Link to relevant study record(s)

Reference

Endpoint:

sediment toxicity: long-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 225 (Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature, protected from moisture

Analytical monitoring:

no

Vehicle:

no

Details on sediment and application:

PREPARATION OF SPIKED SEDIMENT
- Method of mixing: the test item was mixed with quartz sand subsequently the quartz sand was mixed with artificial sediment.
- Equilibration time: 2 days

Test organisms (species):

Lumbriculus variegatus

Details on test organisms:

TEST ORGANISM
- Source: Bundesanstalt fuer Gewaesserkunde (BfG), Koblenz, Germany
- Feeding during test: yes
- Food type: Urtica dioica powder (Heinrich KlenK GmbH & co. KG, Schwebheim, Germany)
- Amount: 0.4 % of sediment dry weight
- Frequency: the plant powder was mixed with the sediment before test start.

Study type:

laboratory study

Test type:

static

Water media type:

freshwater

Type of sediment:

artificial sediment

Duration:

28 d

Exposure phase:

total exposure duration

Hardness:

3.07 mmol/L

Test temperature:

19.4 - 21.5 °C

pH:

7.58 - 8.52

Dissolved oxygen:

6.2 mg/L

Nominal and measured concentrations:

- Nominal test substance concentration: 1000 mg test item/kg sediment dry weight (based on range-finding study)

Details on test conditions:

TEST SYSTEM
- Test container: glass-beakers (6 cm diameter, 250 mL)
- Sediment volume: 45 g, 3cm
- Overlying water volume: 150 mL
- Aeration: yes, slight
- Replacement of evaporated test water, if any: yes

EXPOSURE REGIME
- No. of organisms per container: 10
- No. of replicates per treatment group: 6
- No. of replicates per control / vehicle control: 6
- Type and preparation of food: food was mixed with sediment before test start

CHARACTERIZATION OF ARTIFICIAL SEDIMENT
- Composition: 5% peat, 20% kaolin, 75% quartz sand, 0.47% calcium carbonate
- Moisture: 1.68 %
- pH dry matter and/or whole sediment: 6.85

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day/night regime
- Light intensity: 100-500 lux

EFFECT PARAMETERS MEASURED: mortality and reproduction

TEST CONCENTRATIONS
- Based on a range-finding study
- Test concentrations: 10, 100, 1000 mg/kg sediment dw
- Results used to determine the conditions for the definitive study: no mortality observed

Reference substance (positive control):

yes

Remarks:

potassium chloride

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg sediment dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality, behaviour, biomass, reproduction

Results with reference substance (positive control):

The 28-day NOEC was determined to be 2.0 mg/kg

Validity criteria fulfilled:

yes

Description of key information

With high probability not harmful to sediment.

Additional information

The effect of the substance on sediment organisms was tested in a study according to OECD guideline 225 (BASF SE, 2010). Animals of the species Lumbriculus variegatus were exposed to the substance in a sediment-water system for 28 days. Based on mortality and reproduction a NOEC of 1000 mg/kg sediment dry weight was observed.