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Sediment toxicity

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Reference
Endpoint:
sediment toxicity: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 225 (Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature, protected from moisture
Analytical monitoring:
no
Vehicle:
no
Details on sediment and application:
PREPARATION OF SPIKED SEDIMENT
- Method of mixing: the test item was mixed with quartz sand subsequently the quartz sand was mixed with artificial sediment.
- Equilibration time: 2 days
Test organisms (species):
Lumbriculus variegatus
Details on test organisms:
TEST ORGANISM
- Source: Bundesanstalt fuer Gewaesserkunde (BfG), Koblenz, Germany
- Feeding during test: yes
- Food type: Urtica dioica powder (Heinrich KlenK GmbH & co. KG, Schwebheim, Germany)
- Amount: 0.4 % of sediment dry weight
- Frequency: the plant powder was mixed with the sediment before test start.
Study type:
laboratory study
Test type:
static
Water media type:
freshwater
Type of sediment:
artificial sediment
Duration:
28 d
Exposure phase:
total exposure duration
Hardness:
3.07 mmol/L
Test temperature:
19.4 - 21.5 °C
pH:
7.58 - 8.52
Dissolved oxygen:
6.2 mg/L
Nominal and measured concentrations:
- Nominal test substance concentration: 1000 mg test item/kg sediment dry weight (based on range-finding study)
Details on test conditions:
TEST SYSTEM
- Test container: glass-beakers (6 cm diameter, 250 mL)
- Sediment volume: 45 g, 3cm
- Overlying water volume: 150 mL
- Aeration: yes, slight
- Replacement of evaporated test water, if any: yes

EXPOSURE REGIME
- No. of organisms per container: 10
- No. of replicates per treatment group: 6
- No. of replicates per control / vehicle control: 6
- Type and preparation of food: food was mixed with sediment before test start

CHARACTERIZATION OF ARTIFICIAL SEDIMENT
- Composition: 5% peat, 20% kaolin, 75% quartz sand, 0.47% calcium carbonate
- Moisture: 1.68 %
- pH dry matter and/or whole sediment: 6.85

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day/night regime
- Light intensity: 100-500 lux

EFFECT PARAMETERS MEASURED: mortality and reproduction

TEST CONCENTRATIONS
- Based on a range-finding study
- Test concentrations: 10, 100, 1000 mg/kg sediment dw
- Results used to determine the conditions for the definitive study: no mortality observed
Reference substance (positive control):
yes
Remarks:
potassium chloride
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg sediment dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality, behaviour, biomass, reproduction
Results with reference substance (positive control):
The 28-day NOEC was determined to be 2.0 mg/kg
Validity criteria fulfilled:
yes

Description of key information

With high probability not harmful to sediment.

Key value for chemical safety assessment

Additional information

The effect of the substance on sediment organisms was tested in a study according to OECD guideline 225 (BASF SE, 2010). Animals of the species Lumbriculus variegatus were exposed to the substance in a sediment-water system for 28 days. Based on mortality and reproduction a NOEC of 1000 mg/kg sediment dry weight was observed.

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