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EC number: 223-437-0 | CAS number: 3891-33-6
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1973-12-18 to 1975-03-06
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Scientifically acceptable, method eqivalent or similar to guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 975
- Report date:
- 1975
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- method applied according to BASF-internal standard
- GLP compliance:
- no
Test material
- Reference substance name:
- 1,4-bis(vinyloxy)butane
- EC Number:
- 223-437-0
- EC Name:
- 1,4-bis(vinyloxy)butane
- Cas Number:
- 3891-33-6
- Molecular formula:
- C8H14O2
- IUPAC Name:
- 1,4-bis(ethenyloxy)butane
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Gaukler, 6050 Offenbach/Main, Germany
- Age at study initiation: no data
- Weight at study initiation: 2.7 - 3.0 kg
- Housing: no data
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data
IN-LIFE DATES: From: 1973-12-18 To: 1973-12-27
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- clipped
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated skin of the same animal
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied: ca. 1 mL - Duration of treatment / exposure:
- The test substance was applied for 1 min, 5 min, 15 min and 20 h
- Observation period:
- 8 days
- Number of animals:
- 2
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 x 2.5 cm
- Type of wrap if used:
Application to ear: a cotton ball soaked with test item was fixed to the ear
Application to back: a small piece of cloth was fixed to the back with the help of a gauze patch
REMOVAL OF TEST SUBSTANCE
- Washing: After the short-term applications the test substance was washed off with Lutrol; after the 20-hour application the test substance was not washed off.
- Time after start of exposure: 1 min, 5 min and 15 min
SCORING SYSTEM: observed were the parameters erythema, edema, scaling, and necrosis
Grading system:
"reizlos" Ø: considered as equivalent to Grade 0 (no irritating effect) according to OECD Guideline 404
"fraglich" (+): considered as equivalent to Grade 1 (very slight erythema (barely perceptible)) according to OECD Guideline 404
"leicht" +: considered as equivalent to Grade 2 (well-defined effect) according to OECD Guideline 404
"stark" ++: considered as equivalent to Grade 3 (moderate (to severe)) effect according to OECD Guideline 404
"sehr stark" +++: considered as equivalent to Grade 4 (severe effect) according to OECD Guideline 404
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: incipient scaling after 8 days
- Remarks on result:
- other: 20-hour application to back
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- other: 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: incipient scaling after 8 days
- Remarks on result:
- other: 20-hour application to back
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- other: 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: incipient scaling after 8 days
- Remarks on result:
- other: 20-hour application to back
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: incipient scaling after 8 days
- Remarks on result:
- other: 20-hour application to back
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- other: 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: incipient scaling after 8 days
- Remarks on result:
- other: 20-hour application to back
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- other: 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: incipient scaling after 8 days
- Remarks on result:
- other: 20-hour application to back
- Irritant / corrosive response data:
- Application to ear for 20 hours resulted in Grade 3 erythema and edema after 24 hours. Erythema and edema not reported on day 8, however eschar was observed.
Any other information on results incl. tables
Table of results (20 h application of test item) based on grading system as applied in the study report
time |
animal 1 |
animal 2 |
|
erythema |
24 h |
+ |
+ |
8 d |
Ø |
Ø |
|
edema |
24 h |
+ |
+ |
8 d |
Ø |
Ø |
|
scaling |
24 h |
Ø |
Ø |
8 d |
+ |
+ |
Table of results (20 h application of test item) translated into the grading system as applied in OECD Guideline 404
time |
animal 1 |
animal 2 |
|
erythema |
24 h |
2 |
2 |
8 d |
0 |
0 |
|
edema |
24 h |
2 |
2 |
8 d |
0 |
0 |
|
scaling |
24 h |
0 |
0 |
8 d |
2 |
2 |
Applicant's summary and conclusion
- Interpretation of results:
- slightly irritating
- Remarks:
- Migrated information
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