L-2-hydroxy-2-phenylacetic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study (OECD), validity criteria fulfilled

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

L-2-hydroxy-2-phenylacetic acid

EC Number:

210-276-6

EC Name:

L-2-hydroxy-2-phenylacetic acid

Cas Number:

611-71-2

Molecular formula:

C8H8O3

IUPAC Name:

hydroxy(phenyl)acetic acid

Details on test material:

TS-Freetext:
ChiPros (R)-Mandelsäure: (R)-mandelic acid (purity: 100 %)

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- original source: IRCHA Institut National de Recherche Chimique Appliquée, France, 1978
- age: 2-24 h (starting with the 3rd breed of parent animals)
- no feeding during test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

- total hardness: 2.20-3.20 mmol/l
- alkalinity to pH 4.3: 0.80-1.00 mmol/l
- molar ratio Ca:Mg: ca. 4:1
- conductivity: 550-650 µS/cm

Test temperature:

18-22 °C (max. diff. 2 °C)

pH:

7.5-8.5

Nominal and measured concentrations:

test concentrations: 0 (control), 12.5, 25, 50, and 100 mg/l

Details on test conditions:

- test vessels: glass tubes with flat bottom (20 ml)
- test volume: 10 ml
- loading: 0.5 animals/ml
- 20 animals per concentration level

- illumination: artificial light, type warm white (OSRAM L58 W31)
- day/night rhythm: 16/8 h

- observation times: visually after 0, 24 and 48 h
- observation parameters: swimming ability

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Any other information on results incl. tables

RS-Freetext:
Effect values (mg/l):
            24 h       48 h
EC 0:       >= 100     >= 100

EC 50:      > 100      > 100

EC 100:     > 100      > 100


Immobility:
- all test animals remained mobile throughout the test


Test concentration (retrieval, %):
time   minimum   maximum
(h)
 0    106       114
48    106       106


Test solutions
test conc.    pH             dissolved oxygen (mg/l)
(mg/l)        0 h   48 h     0 h   48 h
control (0)   8.2   8.1      8.8   8.7
 12.5         8.1   8.1      8.7   8.6
 25           8.1   8.1      8.7   8.5
 50           7.2   8.0      8.6   8.5
100           6.5   7.9      8.6   8.4

minimum and maximum values measured in separate vessels (placed close to  test vessels):
- pH value               : 6.5-8.2
- dissolved oxygen (mg/l): 8.4-8.8
- temperature (°C)       : 19.1-19.8

Test medium (M4):
pH value:                8.3
alkalinity up to pH 4.3: 0.88 mmol/l
total hardness:          2.48 mmol/l
conductivity:            621 µS/cm

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In the control the immobilization was ≤ 10%. In the control no daphnids were captured in a surface film. The O2-concentration was > 3 mg/l in all tested replicates. The EC50(24h) of the control substance potassium dichromate (non-GLP) was 1,08 mg/l (Date of the last control experiment: 08 July 2002 project number: 02/0106/50/6). The test is valid.