Reaction mass of 1,4-dichlorobut-2-ene; 3,4-dichlorobut-1-ene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2005

Reference 2

Reference Type:

secondary source

Title:

1,4-Dichlorobut-2-ene - CAS No: 764-41-0

Author:

OECD SIDS

Year:

2006

Bibliographic source:

SIDS Initial Assessment Report for 22th SIAM, UNEP Publications

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Principles of method if other than guideline:

EU Method C.2 is in most parts equivalent to OECD TG 202

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

IUCLID4 Test substance: other TS: 1,4-Dichlorobut-2-ene, purity 96.1 % (31.9 % cis- and 64.2 % trans-isomer)

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.063 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.181 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 0.138-0.237

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.485 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.052 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.156 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.437 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

All results are expressed in terms of mean measured concentrations. Analysis was performed by GC/MS. The recovery rate of the test substance ranged from 2.1 -69.4 % of nominal values in the freshly prepared media after 0 h, and from 2.1 - 27.5 % of nominal values in the media after 24 h of exposure, respectively. Based on the concentrations determined after 0 h of exposure (initial concentrations) the recoveries after 24 h of exposure ranged between 18.6 and 53.6%. Therefore in contrast to the original study in the OECD SIDS (2006) the effect concentrations (EC50, EC0, EC100) are calculated on the basis of the geometric mean.