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Biaccumulation: aquatic/sediments:

The bioconcentration factor (BCF) for chemical 2,4,6-trichloroaniline was determined according static fish test. The BCF value was observed to 27-112 L/kg at dose concentration 0.1 mg/L and 52-147 L/kg at dose concentration 0.01 mg/L on test organism Cyprinus carpio during 8 weeks period. On the basis of BCF values it is concluded that test chemical 2,4,6-trichloroaniline is non bioaccumulative.

Additional information

Biaccumulation: aquatic/sediments:

Various experimental studies have been reviewed from different sources for test chemical 2,4,6-trichloroaniline (CAS no 634-93-5) and their results are summarized below for bioaccumulation in aquatic organisms endpoint.

 

The first experimental study was reviewed from authoritative database (J check, 2018) in this study the Bioaccumulation experiment was conducted for 8 weeks for determination the bioconcentration factor (BCF) of chemical 2,4,6-trichloroaniline (CAS no 634-93-5) on test organism Cyprinus carpio.

The recover ratio used was for test water: 1st concentration area: 88.1 %, 2nd concentration area: 85.0 %, Fish : 92.0 %, and Limit of detection : Test water

1st concentration area: 0.0057 mg/L, 2nd concentration area: 0.00059 mg/L, Fish: 0.04 mg/L. The vehicle used in the study was HCO-20.

Nominal concentrations used in the study are 1st Concentration area: 0. 1 mg/L, 2nd Concentration area: 0.01 mg/L and Range finding study was carried out on Rice fish (Oryzias latipes) TLm(48h) 8.2 ppm(w/v), Thus the bioconcentration factor (BCF) for chemical 2,4,6-trichloroaniline was determined according static fish test. The BCF value was observed to 27-112 L/kg at dose concentration 0.1 mg/L and 52-147 L/kg at dose concentration 0.01 mg/L on the basis of total lipid content on test organism Cyprinus carpio during 8 weeks period. On the basis of BCF values it is concluded that test chemical 2,4,6-trichloroanilineis non bioaccumulative.

 

 Next experiment study was done by Freitag et al (1985) and it is reviewed from journal Chemospher in this study the Bioaccumulation factor of test chemical 2,4,6-trichloroaniline (CAs no. 634-93-5) was determined in fish golden ide (Leuciscus idus melanotus)and it was selected as representative of inhabitants of slightly polluted zones of running water. It represents an intermediate position between trout and carp. Bioconcentration is measured by using the average constant exposition to the chemicals dissolved in water. Bioaccumulation factor of test chemical 2,4,6-trichloroaniline (CAs no. 634-93-5) was determined 330 dimensionless in 3 days. On the basis of BCF value it can be concluded that test chemical is non-bioaccumulative in nature since BCF value does not exceeds 2000 criteria of CLP regulation.

 

Last experimental study was done by W. D .Wolf et al (1993) from journal Archives of Environmental Contamination and Toxicology in this study an experiment was performed to determine Bioaccumulation factor (BCF) of test chemical 2,4,6-trichloroaniline in fish Poecilia reticulate (guppy) for 96 hrs in this experiment guppies (n = 12) were exposed to the test chemical in a static exposure system, where the chemical was dissolved by continuous stirring in autoclaved Cu2+-free Utrecht tap water 4 days prior to the start of experiments. These solutions were further diluted with autoclaved water to obtain the desired exposure concentration. After acclimation to autoclaved water for four days, guppies were exposed in 0.5 L test vessels for 96 h under continuous and gentle aeration with sterile air and the initial concentration of test chemical was 500 nM, the temperature was 23.5 oC, pH of water was between 7-8 and dissolved oxygen concentration of test water was 7 mg/L . Water samples were taken in triplicate every 24 h in order to follow the concentrations of parent compounds. At the end of the experiment, 250 ml of water sample was extracted and were analyzed for possible biotransformation products by gas chromatography with electron capture detection (GC-ECD) and mass spectrometry (GC-MS) (operated in methane/negative ion chemical ionization.

On the basis of analysis the bioaccumulation factor (BCF) of test chemical 2,4,6-trichloroaniline determined to be 1621.81, 199.52 dimensionless on the basis of lipid content and whole body weight respectively. Further, the Uptake rate constant (k1) determined was 68±3 (ww) and 366 ± 63 (lipid) (mt g-1 h-l) and Depuration (loss) rate constant (k2) determined was 0.029 ± 0.007 (h- 1) in fish Poecilia reticulate (guppy) in 96 hrs.

By considering BCF values it is concluded that test chemical 2,4,6-trichloroaniline is non bioaccumulative in nature as the BCF value does not exceed the 2000 criteria of GLP regulation.

 

 On the basis of results of all the studies mentioned above it is observed that Bioaccumulation factor(BCF) of test chemical 2,4,6-trichloroaniline is in range from 27.00 to 1621.81 dimensionless. By considering this range BCF value it can be concluded that test chemical in non bioaccumulative in nature.