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EC number: 263-731-6 | CAS number: 62780-89-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
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- Specific investigations
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- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Skin sensitization
In a Local Lymph Node Assay (LLNA) in mice (CBA/Ca strain) according to OECD Guideline 429, the substance is observed to be sensitising to the skin (Sanders, 2014).
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003-09-29 to 2004-09-23
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- yes
- Remarks:
- limited details on test item, test animals and environmental conditions
- Qualifier:
- according to guideline
- Guideline:
- other: SPL Standard Test method 595.12
- GLP compliance:
- no
- Remarks:
- The study was conducted in a facility operating to Good Laboratory Practice within the UK national GLP monitoring programme but no formal claim of GLP compliance is made for this study.
- Type of study:
- mouse local lymph node assay (LLNA)
- Specific details on test material used for the study:
- No further details on test material next to T1036
- Species:
- mouse
- Strain:
- CBA/Ca
- Sex:
- not specified
- Details on test animals and environmental conditions:
- TEST ANIMALS: no data
ENVIRONMENTAL CONDITIONS: no data
IN-LIFE DATES: no data - Vehicle:
- dimethyl sulphoxide
- Concentration:
- 10%, 25% and 50% w/w in dimethyl sulphoxide
- No. of animals per dose:
- Four animals
- Details on study design:
- RANGE FINDING TESTS:
- Compound solubility: no data
- Irritation: no data
- Lymph node proliferation response: no data
-There were no signs of systemic toxicity following a preliminary sighting test at a concentration of 50%.
MAIN STUDY
ANIMAL ASSIGNMENT AND TREATMENT
- Name of test method: SPL Standard Test Method 595.12
- Criteria used to consider a positive response: Test to control ratio greater than 3.0 indicates a ‘positive’ result.
TREATMENT PREPARATION AND ADMINISTRATION: Three groups, each of four animals, were treated with 50 uL of the test material (25 uL per ear) as asolution in dimethyl sulphoxide at concentrations of 10%, 25% and 50% w/w. A further group of four animals was treated with dimethyl sulphoxide alone. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- no data
- Positive control results:
- see: any other information on results (here below)
- Parameter:
- SI
- Value:
- 0.92
- Test group / Remarks:
- 10% w/w group
- Parameter:
- SI
- Value:
- 1.25
- Test group / Remarks:
- 25% w/w group
- Parameter:
- SI
- Value:
- 4.05
- Test group / Remarks:
- 50% w/w group
- Parameter:
- EC3
- Value:
- 40.63
- Cellular proliferation data / Observations:
- CELLULAR PROLIFERATION DATA
no data
DETAILS ON STIMULATION INDEX CALCULATION
The Stimulation Index (SI) was expressed as the mean radioactive incorporation for each treatment group divided by the mean radioactive incorporation of the vehicle control group
EC3 CALCULATION
Using the data generated, an EC3 value of 40.63% was calculated.
CLINICAL OBSERVATIONS:
no signs of systemic toxicity in a preliminary sighting test. No data for the main study
BODY WEIGHTS
no data - Interpretation of results:
- Category 1B (indication of skin sensitising potential) based on GHS criteria
- Conclusions:
- The test material was considered to be a sensitiser under the conditions of the test. Using the data generated, an EC3 value of 40.63% was calculated.
Reference
Table 1: The Stimulation Index (SI) expressed as the mean radioactive incorporation for each treatment group divided by the mean radioactive incorporation of the vehicle control group are as follows:
Concentration (% w/w) in dimethyl sulphoxide | Stimulation Index (SI) b | Result |
10 | 0.92 | Negative |
25 | 1.25 | Negative |
50 | 4.05 | Positive |
Using the data generated, an EC3 value of 40.63% was calculated.
b = Test to control ratio greater than 3.0 indicates a ‘positive’ result
Table 2: Positive Control Local Lymph Node Assay in the Mouse (2003)
Start Date |
Finish Date |
Test Material |
Concentration |
Vehicle |
Stimulation Indexa |
Classificationb |
13/03/2003 |
19/03/2003 |
α‑Hexylcinnamaldehyde, tech., 85% |
5%, 10%, 25% v/v |
acetone/olive oil 4:1 |
2.8, 2.3, 5.5 |
Positive |
13/03/2003 |
19/03/2003 |
α‑Hexylcinnamaldehyde, tech., 85% |
5%, 10%, 25% v/v |
acetone/olive oil 4:1 |
2.0, 1.9, 6.8 |
Positive |
10/10/2003 |
16/10/2003 |
α‑Hexylcinnamaldehyde, tech., 85% |
5%, 10%, 25% v/v |
acetone/olive oil 4:1 |
1.76, 2.78, 5.06 |
Positive |
16/10/2003 |
22/10/2003 |
α‑Hexylcinnamaldehyde, tech., 85% |
5%, 10%, 25% v/v |
acetone/olive oil 4:1 |
1.49, 1.73, 5.26 |
Positive |
a= Ratio of test to control lymphocyte proliferation
b= Stimulation index greater than 3.0 indicates a positive result
* = Standard Test Method 595 (Pooled nodes)
·= Standard Test Method 599 (Individual nodes)
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (sensitising)
- Additional information:
Sanders (2004) investigated the skin sensitization potential of T001036 following topical application to the dorsal surface of the ear of CBA/Ca mice. Primary lymphocyte proliferation was assessed during the sensitizing (induction) phase of the response. Following a preliminary sighting test at which there were no signs of systemic toxicity at a concentration of 50%, three groups, each of four animals, were treated with 50 µL of the test item (25 µL per ear) as a solution in dimethyl sulphoxide at concentrations of 10%, 25% and 50% w/w in dimethyl sulphoxide. A further group of four animals was treated with dimethyl sulphoxide alone. The following stimulation index (SI) values were observed: at 10% concentration: 0.92 (negative result), at 25%: 1.25 (negative result) and at 50%: 4.05 (positive result as test to control ratio was greater than 3.0). Using the data generated, an EC3 value of 40.63% was calculated.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
Skin sensitization
The test item was considered to be a sensitiser under the conditions of the test. Using the data generated, an EC3 value of 40.63% was calculated. Based on these data and the criteria of the CLP Regulation (EC3 value > 2%), the substance is classified as skin sensitizer category 1B (H317: May cause an allergic skin reaction).
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