4-amino-5-hydroxynaphthalene-1,7-disulphonic acid

Toxicity to microorganisms

Description of key information

The toxicity to microorganism was estimated by the QSAR Toolbox version 3.3 prediction the 48 hours IGC50(Inhibition growth concentration) was estimated to be 823.090393066 mg/l for test substance  4-amino-5-hydroxynaphthalene-1,7-disulphonic acid with growth inhibition effects. 

Additional information

Toxicity to microorganisms study for Target -7-Amino-1-hydroxy-3-naphthalenesulfonic acid(Cas no.130-23-4)Summarized as fallowed:

 

From SSS QSAR prediction model, 2016 for target chemical:

The toxicity to microorganism was estimated by the QSAR Toolbox version 3.3 prediction,in freshwater and semi static condition the 48 hours IGC50(Inhibition growth concentration) was estimated to be 823.090393066 mg/l for test substance 4-amino-5-hydroxynaphthalene-1,7-disulphonic acid with growth inhibition effects.

This predicted IGC50 value indicate that the test substance 4-amino-5-hydroxynaphthalene-1,7-disulphonic acid is non hazardous to aquatic microorganisms.

Other peer reviewed summary (GREIM H.,et al;Chemosphere. Vol. 28, No. 12, pp. 2203.2236, 1994) of read across chemical 7-Amino-4 hydroxy- 2 – naphthalenesulphonic (Cas no.87-02-5) as followed:

The investigation was performed to determine the toxicity of sulphonic acids to bacteria.

Based on different test procedures, The EC0 for 7-Amino-4 hydroxy- 2 – naphthalenesulphonic Acid was determined to be 1000 mg/l on the basis of inhibition effect.

Based on the no effect concentration value the test chemical 7-Amino-4 hydroxy- 2 - naphthalenesulphonic Acid can be considered non- toxic to bacteria (i.e microorganisms).

 

Another result from experimental study- Journal of Scientific & Industrial Research, Vol. 70, Pg. no. 525-532, July 2011. Of read across substance (Cas no.90-51-7):

Toxicity to micro-organisms study was conducted on gram positive bacteria (Staphylococcus aureus,Bacillus subtilis), gram negative bacteria (Escherichia coliandPseudomonas aeruginosa) and fungiCandida albicans.

 

The antibacterial assay was performed using Kirby – Bauer disk diffusion method. MIC was determined for all the different test organisms.

 

Test chemical was dissolved in DMF (< 500 ppm conc.). The test substance conc. used for the study was 20, 50, 100, 200, 300, 500, and 1000 mg/l, respectively.

 

The melting point of test chemical was determined in PMP-DM scientific melting point apparatus. Purity of test chemical was determined by thin layer chromatography (TLC) using silica gel-G coated Al-plates (0.5 mm thickness).

 

Infrared spectra was recorded on Shimadzu FT - IR 8400S model using KBr pellets.¹H NMR spectra was acquired on a Varian 400 MHz model spectrophotometer using DMSO as a solvent and TMS as internal reference (chemical shifts in ‘, ppm).

 

Elemental analysis of C, H, and N were carried out on Carlo Erba 1108 instrument.

 

For antibacterial and antifungal assay, ciprofloxacin and flucanazole was used as a standard dyes.

The melting point of test chemical was found to be > 300°C.

  The MIC value for 5 different organism’s are-

Pseudomonas aeruginosa– 1000 mg/l,Staphylococcus aureus– 300 mg/l ,Escherichia coli– 300 mg/l ,Bacillus subtilis– 100 mg/land Candida albicans– 300 mg/l

All above MIC values for different species i.e gram positive bacteria (Staphylococcus aureus,Bacillus subtilis), gram negative bacteria (Escherichia coliandPseudomonas aeruginosa) and fungiCandida albicansindicate that the test substance is non toxic to microorganisms.

Overall weight of evidence studies from prediction model for target chemical (Cas no.130-23-4) and peer reviewed journals of read across substances (Cas no.87-02-5 and 90-51-7) indicate that the chemical 7-Amino-1-hydroxy-3-naphthalenesulfonic acid is non toxic to micro-organism and thus not consider for further classification.