2-Propenoic acid, 2-methyl-, 2-[2-(4-benzoylphenoxy)ethoxy]ethyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline Study with detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

MAEEBP

IUPAC Name:

MAEEBP

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): MAEEBP- Physical state: colourless liquid- Composition of test material, percentage of components: 4-methacryloyl diethoxy benzophenone 85 area%, impurities 4-hydroxybenzophenone <5%, 4-methacryloyl benzophenone <5%, polymethacrylate <5%- Lot/batch No.: 150301- Expiration date of the lot/batch: 31. Mar.. 2016- Storage condition of test material: fridge: 2-8°C, keep away from light and humidity

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: Corresponding amount added to dilution water and shaked vigorously for 23 hours. The resulting solution was filtrated through 0.45 µm nylon filters. The filter was rinsed with an aliquot of the respective water-accommodated fraction (WAF) before filling the test vessel. Then the water-accommodated fraction (WAF) was filtered in a glass beaker. This solution was transferred into the 4 replicates of the test vessels.- Dilution water specification: CaCl2*2H2O: 293.80 mg/LMgSO4*7H2O: 123.30 mg/LNaHCO3: 64.80 mg/LResulting hardness in mmol/L: 2.502Resulting hardness in mg CaCO3/L: 250

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: Daphnia magna - Strain: STRAUS, Berlin- Source: Umweltbundesamt Berlin- Age at study initiation: <24 h- Method of breeding: Daphnia magna is bred in the LAUS GmbH throughout the year. The husbandry is performed similar to the method described in EN ISO 6341, following SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), Version 12, valid from 02. Feb. 2015.- Feeding during test: no- Food type: unicellular green algae (Desmodesmus subspicatus)

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg CaCO3/L

Test temperature:

19.0 – 21.0 °C

pH:

7.6 - 7.7

Dissolved oxygen:

7.5 - 8.9 mg/L

Nominal and measured concentrations:

2.2, 4.6, 10, 22, 46 mg/L (nominal concentrations)Because of the limited solubility in test medium the correlation between nominal and measured concentration in the 3 highest concentrated treatments was weak. The correlation of the measured concentrations within the test concentrations was very good.The determination of the results was based on the geometric mean of the measured concentrations.

Details on test conditions:

TEST SYSTEM- Test vessel: Beakers, glass, nominal volume 50 mL- Fill volume: 20 mL- Aeration: After preparation, the dilution water was aerated- Renewal rate of test solution: 24 h- No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4- No. of vessels per control (replicates): 4TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Deionised water with an enrichment of certain minerals (as demanded in the guidelines)- Intervals of water quality measurement: pH, the concentration of dissolved oxygen and the content of the test item in the test vessels were measured at the beginning and at the end of the test and at medium renewal in the old and the new test solutionOTHER TEST CONDITIONS- Adjustment of pH: no- Photoperiod: 16/8 hours

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: CI not determinable

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

14 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI 13 - 17 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

7.9 g/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? yes- 24h-EC50: 1.6 mg/L

Reported statistics and error estimates:

The estimation of the biological results was accomplished using the software ToxRat® Professional, version 3.2.

Any other information on results incl. tables

Immobility:

Nominal Concentration in mg/L	Immobility 24 hours					Immobility 48 hours
	absolute				in %	absolute				in %
Blank control	0	0	0	0	0	0	0	0	0	0
2.2	0	0	0	0	0	0	0	0	0	0
4.6	0	0	0	0	0	0	0	0	0	0
10	0	0	0	0	0	4	4	2	3	65
22	2	2	3	2	45	5	5	5	5	100
46	4	3	2	1	50	5	5	5	5	100

 

Measured Concentrations

Nominal Concentration Test Item	Measured Concentration  t = 0 h	Measured Concentration  t = 24 h old	Measured Concentration t = 24 h new	Measured Concentration t = 48 h	Geometric Mean
mg/L	mg/L	mg/L	mg/L	mg/L	mg/L
Blank control	< LOQ	< LOQ	< LOQ	< LOQ	--
2.2	1.96	2.03	2.15	2.18	2.08
4.6	3.94	4.01	4.00	4.08	4.01
10	7.95	8.09	7.60	7.90	7.88
22	12.72	13.43	11.99	12.46	12.64
46	14.45	14.35	14.19	14.87	14.46

LOQ (Limit of quantification) = 0.5 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The short term toxicity of MAEEBP against Daphnia magna STRAUS was tested according to OECD 202 resp. EU C.2. The 48 h-EC50 value, based on the geometric mean of the measured concentrations, was determined to be 7.6 mg/L.

Executive summary:

The short term toxicity of MAEEBP against Daphnia magna STRAUS was tested according to OECD 202 resp. EU C.2. For each test concentration, 20 Daphnia were exposed to the test item (46 / 22 / 10 / 4.6 / 2.2 mg) for 48 hours in a semi static test system. After 24 and 48 hours, the immobilised Daphnia were counted. 3 concentrations showed toxicity between 65 and 100% immobilisation after 48 hours. None of the animals were immobilised in the control. Potassium dichromate K₂Cr₂O₇(CAS No. 7778-50-9) was used as positive control in a current reference study.The 24h-EC50 value was determined as 1.6 mg/L, lying within the demanded range of 0.6 – 1.7 mg/L stated in the EU guideline.

The concentration of the test item in the test solutions was determined using HPLC measurement at the start and at the end of the test and at medium renewal after 24 hours. Because of the limited solubility in test medium the correlation between nominal and measured concentration was weak and the measured concentrations in the higher concentrated treatments were in a similar range. Therefore, the determination of the results was based on the geometric mean of the measured concentrations.

The following results were determined for the test item MAEEBP(species:Daphnia magna). 

48h-NOEC = 4.0 mg/L
48h-LOEC = 7.9 mg/L
24h-EC50 = 14 mg/L
48h-EC50 = 7.6 mg/L