2-methylpyridine

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Similar to guideline study but taking place prior to establishment of guideline and GLP.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylpyridine

EC Number:

203-643-7

EC Name:

2-methylpyridine

Cas Number:

109-06-8

Molecular formula:

C6H7N

IUPAC Name:

2-methylpyridine

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Test system

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

0.1 ml undiluted, placed in conjunctival sac of right eye

Duration of treatment / exposure:

15 min and 24 h

Observation period (in vivo):

14 days

Number of animals or in vitro replicates:

3

Details on study design:

The treated eye of the rabbits was washed with physiological saline at 15 minutes and at 24 hour after exposure. Evaluations were made according to the Draize scale. Animals were evaluated for 14 days after treatment

Results and discussion

In vivo

Results

Irritation parameter:

maximum mean total score (MMTS)

Remarks:

according to Draize, Woodard and Calvery, 1944

Basis:

mean

Time point:

other: 24 h

Score:

71.6

Max. score:

110

Reversibility:

fully reversible within: 14 days

Irritant / corrosive response data:

Observations at 1 and 24 h after exposure: moderate to severe erythema and edema, slight edema, copious discharge, translucent areas of corneal cloudiness, iris unresponsive to light.
After 168 hours: diffuse to translucent areas of corneal cloudiness, slight erythema in one instance; no edema or discharge.
After 10 days: rarely perceptible areas of corneal cloudiness in two instances.
After 14 days: eyes normal. zero readings.

Other effects:

Improvement throughout the observation period, with no abnormalities noted at 14 days post treatment.

Any other information on results incl. tables

EYE IRRITATION IN RABBITS AFTER APPLICATION OF

 0.4 MOLE Fraction 2-Methylpyridine

Sample (0.1 ml) Applied Undiluted. Irrigated after 24-hour Exposure

 

Animal No., Sex	1 hour	24 hours	48 hours	72 hours	120 hours	168 hours
1 female	58	66	64	50	44	40
2 male	64	71	67	50	40	27
3 female	56	76	70	68	38	30
Average	59.3	71.0	67.0	49.3	40.6	32.3

 

 

Sample (0.1 ml) Applied Undiluted. Irrigated after 15-Minute Exposure

 

Animal No., Sex	1 hour	24 hours	48 hours	72 hours	120 hours	168 hours
1 female	68	69	30	24	7	0
2 male	66	49	24	22	10	0
3 female	60	56	35	20	5	0
Average	64.6	57.3	29.6	22.0	7.3	0.0

 

Interpretation of Eye Irritation scores:

0 -0.5: non-irritating

0.6 -2.5: practically non-irritating

1.6 -15: minimally

15.1 -25: mildly

25.1 -50: moderately

50.1 -80: severely

80.1 -100: extremely

100.1 -110: maximally

Applicant's summary and conclusion

Interpretation of results:

highly irritating

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

4-Methylpyridine was tested in a Draize eye irritation protocol in rabbits, with the conclusion that the substance is a severe eye irritant. Corneal cloudiness observed after the exposure resolved in 14 days.