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EC number: 247-668-1 | CAS number: 26402-26-6
- Life Cycle description
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- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
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- Endpoint summary
- Stability
- Biodegradation
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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- Long-term toxicity to aquatic invertebrates
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Jul - 25 Aug 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt Baden-Württemberg, Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal wastewater treatment plant AZV Breisgauer Bucht (predominantly domestic wastewater), sampling date of activated sludge: 26 July 2022,
- Preparation of inoculum for exposure: The activated sludge was washed twice with tap water by settling the sludge, decanting the supernatant and re-suspending the sludge. The sludge was aerated at ambient temperature until use.
- Concentration of sludge: 30 mg d.s./L
- Dry solids concentration: 4.27 g/L (drying at 105°C for 5 hours)
- Water filtered: no - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- COD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- BOD was determined and biodegradation in % chemical oxygen demand COD was calculated
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to the guideline
- Solubilising agent: none
- Test temperature: 21.3 – 21.5°C throughout the whole study and therefore within the required range of 22 ± 2°C
- pH: 7.5 (at the beginning and termination of test)
- pH adjusted: The pH of the mineral medium was 7.7 and was therefore adjusted to 7.5 with HCl. After preparation of test solutions, no pH adjustment was needed (pH: 7.5)
- Aeration of dilution water: yes, at least half an hour
- Susspended solids concentration: 30 mg d.s./L
TEST SYSTEM
- Culturing apparatus: OxiTop® (WTW / Xylem)
- Number of culture flasks/concentration: 3 replicates
- Method used to create aerobic conditions: inoculum was aerated until use (see details on inoculum and medium/test solutions were prepared under oxic conditions)
- Measuring equipment: Sensomat (AQUALYTIC®)
- Test performed in closed vessels: yes
- Test performed in open system: no
- Details of trap for CO2 if used: bottles were equiped with rubber quiver inserts for NaOH pellets, which absorb the carbon dioxide produced.
SAMPLING
- Sampling frequency and method: Every 112 minutes, the current pressure was measured and stored by each measuring head.
- Sterility check if applicable: not applicable
- Sample storage before analysis: no
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 replicates (only inoculum)
- Reference substance: 3 replicates (concentration corresponding to 99.8 mg COD/L)
- Toxicity control: 1 replicate (48.1 mg/L test item and 21 mg reference substance corresponding to a concentration of 200 mg/L COD) - Reference substance:
- acetic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- COD (chemical oxygen demand)
- Value:
- 85.3
- Sampling time:
- 28 d
- Details on results:
- A degradation of >60% was reached on Day 6 and therefore within a 10-d window.
The degradation of the toxicity control after 14 days was >25%. Therefore, the test item had no inhibitory effect on the inoculum according to the criterion of the guideline.
The oxygen consumption of the blanks after 28 days was 20.6 mg/L. - Results with reference substance:
- The reference compound sodium acetate reached the pass level of 60% COD within 5 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- For details refer to field "any other information on results incl. tables"
- Interpretation of results:
- readily biodegradable
Reference
Table 1: Biodegradation in % of COD
|
Test item |
Tox control |
Reference item |
||||
Test item* [mL/164 mL] |
164 |
164 |
|
||||
Reference item** [mL or mg/164 mL] |
|
21 |
164 |
164 |
164 |
||
COD [mg/L] |
100 |
100 |
100 |
199 |
99.8 |
99.8 |
99.8 |
Day |
Degradation [%] |
||||||
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
4 |
49.6 |
49.6 |
49.6 |
48.9 |
58.2 |
58.2 |
55.4 |
8 |
70.3 |
67.5 |
67.5 |
69.1 |
73.2 |
73.2 |
70.4 |
12 |
80.6 |
77.8 |
77.8 |
78.1 |
80.8 |
80.8 |
78.0 |
16 |
81.3 |
78.8 |
78.8 |
84.7 |
81.4 |
81.4 |
81.4 |
20 |
82.4 |
82.4 |
82.4 |
86.2 |
82.6 |
82.6 |
82.6 |
24 |
86.3 |
83.3 |
83.3 |
88.2 |
83.5 |
83.5 |
83.5 |
28 |
86.3 |
86.3 |
83.3 |
88.2 |
83.5 |
86.5 |
86.5 |
*stock solution of 128.0 mg/L
**reference vessels: stock solution of 128.0 mg/L, toxicity control: direct addition
Table 2: Validity criteria for OECD 301 F.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
less than 20% |
yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
pass level of 60% COD reached within 5 days |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
78.1 % on Day 12 |
yes |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days. |
was within the range of 20 – 30 mg O2/L |
yes |
Description of key information
Readily biodegradable: 85.3% biodegradation after 28 d based on COD (OECD 301 F)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
One GLP study according to the OECD guideline 301 F is available testing the ready biodegradability of the substance Octanoic acid, monoester with glycerol (CAS 26402-26-6). The study was conducted using as inoculum activated sludge from a wastewater treatment plant treating predominantly domestic wastewater. A toxicity control (with 48.1 mg test item/L and 21 mg/L reference item, corresponding to 199 mg/L COD) and a reference control were run in parallel. The substance was found to be readily biodegradable with a biodegradation of 85.3% based on chemical oxygen demand (COD) after 28 d. A degradation of > 60% was reached on Day 6 and therefore within a 10-d window. Based on the results from the toxicity control (>25% degradation already on Day 4) the test item had no inhibitory effect on the inoculum. The reference compound sodium acetate reached the pass level of 60% COD within 5 days. All validity criteria set out in the guideline were fulfilled and therefore the study is reliable.
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