Trifluoroacetic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: A reliable and scientifically sound guideline study referring to a read-across substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sodium trifluoroacetate

EC Number:

220-879-6

EC Name:

Sodium trifluoroacetate

Cas Number:

2923-18-4

Molecular formula:

C2HF3O2.Na

IUPAC Name:

sodium trifluoroacetate

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Molecular weight: 136 g/mol
- Water solubility: 625 g/L at 25°C
- Appearance: white powder

Based on the molecular weights, a concentration of 1200 mg/L sodium trifluoroacetate/L corresponds to 1000 mg trifluoroacetate anion/L.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 and 1200 mg/L (nominal) in ISO water
- Sampling: 0h and 48h after test initiation
- Sample storage conditions before analysis: the samples were stored at cool and dark before analysis

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in house culture at Solvay Duphar B.V. since August 1991 and originates from RIVM, The Netherlands
- Age at study initiation: not older than 24 hours
- Feeding during test: no

BREEDING
- Method of breeding: cultured in vessels with 2L reconsituted water (ISO), prepared and aerated at least 24h prior to use
- Feeding during breeding: algal suspension + yeast three times a week
- Culture chamber conditions
- Temperature: 20 ± 1°C
- Illumination: 16 h/d
- Age of Daphnia: not older than 4 weeks

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

19.4 - 20.5°C

pH:

7.7 - 8.0

Dissolved oxygen:

8.4 - 8.6 mg/L

Nominal and measured concentrations:

Nominal: 1200 mg/L and control
Measured: 1220 mg/L (at test start) and 1210 mg/L (at test end)

Details on test conditions:

TEST SYSTEM
- Test vessel: beaker glasses
- Type: open
- Material, size, fill volume: glass, 250 mL (size), 200 mL (fill volume)
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: reconstitued water (ISO)
Deionised water (LRS 1322 830 78115), 294 mg/L CaCl2*2H2O, 123 mg/L MgSO4*7H2O, 64 mg/L NaHCO3, 5.8 mg/L KCl (pH = 7.8; hardness (CaCO3 = ca. 250 mg/L))
- Intervals of water quality measurement: at the beginning (0h) and at the end of the study (48h)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h/light, 8h/dark

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 10, 30, 100, 300, 1000 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50 (48h): 0.27 mg/L