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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with German national standards
Qualifier:
according to
Guideline:
other: DIN 38412 part 9
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: SAG 86.81
- Method of cultivation: in OECD medium
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
20 °C
Nominal and measured concentrations:
Nominal test concentrations: control, 15.625, 31.25, 62.5, 125, 250, 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, fill volume: Erlenmeyer glass flasks, 250 ml
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates, inoculated): 4
- No. of vessels per concentration (replicates, uninoculated): 2
- No. of vessels per control (replicates, inoculated): 4
- No. of vessels per control (replicates, uninoculated): 2

GROWTH MEDIUM
- Standard medium used: yes(OECD medium)

EFFECT PARAMETERS MEASURED: measured after 0 ,24 ,48, 72 hours
- Determination of cell concentrations: fluorimeter

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 130 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate
Validity criteria fulfilled:
not specified

Description of key information

EC50(72h) > 130 mg/L (nominal, active ingredient) for Desmodesmus subspicatus ( DIN 38412 part 9)

Key value for chemical safety assessment

Additional information

One study investigating the toxicity of the test substance to aquatic algae is available. The study was performed according to the German national standard DIN 38412 using Desmodesmus subspicatus as test organism (BASF 1989). Concentrations between 4 and 130 mg a.i./L. After an exposure time of 72h no treatment showed growth inhibition of >= 50%. Thus, the 72h-EC50 is determined to be > 130 mg a.i./L based on nominal concentrations.