Reaction mass of 1-(2,6,6-trimethylcyclohex-2-en-1-yl)hepta-1,6-dien-3-one and 1-(2,6,6-trimethylcyclohex-1-en-1-yl)hepta-1,6-dien-3-one

Endpoint summary

Description of key information

Acute oral toxicity LD50 in mice > 9500 mL/kg bw (8836 mg/kg bw)

Acute inhalation toxicity LC50 route to route extrapolation > 23000 mg/kg bw

Acute dermal toxicity LD50 in rabbits > 5000 mg/kg bw

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1955

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Study was performed predating current guidelines

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

5 day observation period

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Species:

mouse

Strain:

CF-1

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Carworth
- Housing: Individual cages
- Diet: Regular diet of Fox Blox

Route of administration:

oral: gavage

Vehicle:

other: Distilled water plus Tween 20 to aid in mixing

Details on oral exposure:

VEHICLE
- Actual amount of test material fed: 0.1, 0.14, 0.16, 0.18, 0.20, 0.30, and 0.40 mL corresponding with 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively.
- Justification for choice of vehicle: distilled water and Tween 20 to aid mixing.

DOSAGE PREPARATION
The test material was diluted one part plus four parts with diluted water plus Tween 20.

Doses:

5, 7, 8, 9, 10, 15, and 20 mL/kg

No. of animals per sex per dose:

5, 5, 10, 10, 10,10, and 5 animals (sex unspecified) in the 5, 7, 8, 9, 10, 15, and 20 mL/kg dose goups, respectively.

Control animals:

no

Details on study design:

- Duration of observation period following administration: 5 days

Key result

Sex:

not specified

Dose descriptor:

LD50

Effect level:

9 500 mL/kg bw

Based on:

test mat.

Remarks on result:

other: LD50 calculated with Behrens method

Mortality:

0/5, 0/5, 1/10, 4/10, 7/10, 9/10, and 5/5 animals died within the 5 day observation period after exposure to 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively.

Interpretation of results:

other: Not classified

Remarks:

According to Regulation (EC) No. 1272/2008.

Conclusions:

The acute oral toxicity test showed an LD50 of 9500 mL/kg bw

Executive summary:

A preguideline study was performed to identify the acute oral toxicity of the test substance in mice. In this study, 5, 5, 10, 10, 10,10, and 5 mice (sex unspecified) were administered with 5, 7, 8, 9, 10, 15, and 20 mL/kg . substance at dose levels of 5, 7, 8, 9, 10, 15, and 20 mL/kg bw. 0/5, 0/5, 1/10, 4/10, 7/10, 9/10, and 5/5 animals died within the 5 day observation period after exposure to 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively. Under the conditions of the test, the acute oral LD50 for the substance in mice was determined to be 9500 mL/kg bw (calculated with Behrens method).

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Acute toxicity: via inhalation route

Link to relevant study records

Reference

Endpoint:

acute toxicity: inhalation

Data waiving:

other justification

Justification for data waiving:

other:

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Acute toxicity: via dermal route

Link to relevant study records

Reference

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Study is carried out predating current guidelines

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Species:

rabbit

Strain:

not specified

Sex:

not specified

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24 hours

Doses:

5000 mg/kg

No. of animals per sex per dose:

3 (intact skin)
3 (abraded skin)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days

Key result

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality was observed within the 14 day observation period.

Other findings:

Dry cracked skin was noted.

Interpretation of results:

other: Not classified

Remarks:

According to Regulation (EC) No. 1272/2008.

Conclusions:

The acute dermal toxicity test showed an LD50 > 5000 mg/kg bw

Executive summary:

A preguideline study was performed to identify the acute dermal toxicity of the test substance.In this study 3 albino rabbits were administered with 5000 mg/kg neat test substance on the intact skin. The animals were exposed for 24 hours under occlusive conditions. No mortality was observed within the 14 -day observation period. Under the conditions of the test, the acute dermal LD50 for the substance in rabbits was > 5000 mg/kg bw.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Dose descriptor:

discriminating dose

Value:

5 000 mg/kg bw

Additional information

Oral

A preguideline study was performed to identify the acute oral toxicity of the test substance.In this study,5, 5, 10, 10, 10,10, and 5 mice (sex unspecified) were administered with 5, 7, 8, 9, 10, 15, and 20 mL/kg substance at dose levels of 5, 7, 8, 9, 10, 15, and 20 mL/kg bw. 0/5, 0/5, 1/10, 4/10, 7/10, 9/10, and 5/5 animals died within the 5 day observation period after exposure to 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively. Under the conditions of the test, the acute oral LD50 for the substance in mice was determined to be 9500 mL/kg bw (calculated with Behrens method). With a density of 0.9301 this equals to 8836 mg/kg bw.

Dermal

A preguideline study was performed to identify the acute dermal toxicity of the test substance.In this study 3 albino rabbits were administered with 5000 mg/kg neat test substance on the intact skin. The animals were exposed for 24 hours under occlusive conditions. No mortality was observed within the 14 -day observation period. Under the conditions of the test, the acute dermal LD50 for the substance in rabbits was > 5000 mg/kg bw.

Inhalation:

The acute inhalation toxicity for the substance can be derived using data on the acute oral toxicity using the following methodology*: CLP guidance (2015 3.1.6.1.8. Example 8, page 268): using the extrapolation formula 1 mg/kg bw = 0.0052 mg/L/4h. The LD50 of the substance for acute oral toxicity is 8836 mg/kg bw. This 8836 mg/kg bw can be converted to 45.9 mg/L = 45.9 gram/m3. Taking into account the inhalation absorption as 100% and oral absorption 50%, the acute inhalation toxicity would become 23.0 g/m3 = 23000 mg/m3. The maximum saturated vapour pressure for Hexalon is 685 mg/m3 (0.08 Pa (Vap Pr. Hexalon) x232(MW) / 8.3 (R, gas constant) x 297 (°K)). This means that Hexalon cannot reach a concentration higher than 685 mg/m3. The extrapolated inhalation LC50 cannot be reached because it exceeds the saturated vapour pressure by more than a factor of 33.

*The equation is based on rat inhalation volume while the LD50 is from mice. Even if the mice inhalation volume would be twice that of rat the LC50 inhalation would much exceed the SVP.

Justification for classification or non-classification

Based on the available information classification and labelling for acute oral, dermal and inhalation toxicity is not warranted in accordance with EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation No. 1272/2008 and its amendments.