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Reaction products of diazotised 2-amin-6-nitrophenol-4-sulphonic Acid and 2-Amino-4-nitrophenol-6-sulfonic Acid, coupled with a mixture of 6-Amino-4-hydroxy-2-naphthalenesulfonic Acid and Acetylated 6-Amino-4-hydroxy-2-naphthalenesulfonic Acid, subsequently metallized with Chromium (III) Basic Sulfate and Acetic Acid, and at the end diazotised and coupled with p-Cresol, sodium salts
EC number: 405-750-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- May 12, 1981
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 405-750-6
- EC Name:
- -
- Molecular formula:
- Not applicable for UVCB substance
- IUPAC Name:
- diiron(3+) dihydrogen tris(7-[2-(2-hydroxy-5-methylphenyl)diazen-1-yl]-2-[2-(3-nitro-2-oxido-5-sulfophenyl)diazen-1-yl]-3-sulfonaphthalen-1-olate) 7-acetamido-2-[2-(3-nitro-2-oxido-5-sulfophenyl)diazen-1-yl]-3-sulfonaphthalen-1-olate
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Remarks:
- SPF-Quality
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
-Source: Broekman Institute, Someren / The Netherlands
-Age at start of treatment: approx. 13 weeks
-Body weight at start of treatment: 2515-2882 g
-Identification: ear tag
-Acclimatisation: at least five days under test conditions
-Housing: individual cages with perforated floors equipped with an automatic drinking system (ITL, Bergen / The Netherlands).
-Diet: standard laboratory rabbit diet (LKK-20, pellet diameter 4mm, Hope Farms, Woerden / The Netherlands), approx. 100 g per day
-Water: tap-water diluted with decalcified water, ad libitum.
ENVIRONMENTAL CONDITIONS
-Temperature: 21 ± 3°C
-Humidity: 30-70 %
-Air changes: 7.5-15 air changes per hr with air-conditioned
-Photoperiod: 12 hrs artificial fluorescent light / 12 hrs dark.
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- clipped
- Vehicle:
- water
- Remarks:
- milli-RO water (water prepared by reverse osmosis)
- Controls:
- not specified
- Amount / concentration applied:
- TEST MATERIAL
-Amount applied: 0.5 g/animal
-Preparation: moistened with milli-RO water (water prepared by reverse osmosis) - Duration of treatment / exposure:
- 4 hours of application
- Observation period:
- 40 min, 24, 48 and 72 h after the removal of the dressings
- Number of animals:
- 3 females
- Details on study design:
- TREATMENT
Approximately 24 h before treatment, the dorsal fur was shaved with an electric clipper, exposing an area of approximately 100 cm2 (10 cm * 10 cm).
Prior to test substance administration, the animals were physically examined and the skin found to be intact and normal.
The test substance was applied to the intact skin of the shaved area, using a Metalline patch (2 * 3 cm) mounted to Micropore tape. The dressing was wrapped around the abdomen and anchored with an elastic bandage.
REMOVAL OF TEST SUBSTANCE
The remaining test substance was removed using a dry tissue and subsequently a tissue moistened with tap-water.
OTHER OBSERVATIONS
-Viability / Mortality, daily.
-Body Weight, once (application day)
-Staining of the treated skin area related to the test substance was described and recorded during each observation.
SCORING SYSTEM
-Erythema and Eschar Formation
No erythema:0
Very slight erythema (barely perceptible): 1
Well defined erythema: 2
Moderate to severe erythema: 3
Severe erythema (beet redness) to slight eschar formation (injuries in depth): 4
-Oedema Formation
No oedema: 0
Very slight oedema (barely perceptible): 1
Slight oedema (edges of area well defined by definite raising): 2
Moderate oedema (raised approximately 1 mm): 3
Severe oedema (raised more than 1 mm and extending beyond area of exposure): 4
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0.3
- Max. score:
- 1
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Irritant / corrosive response data:
- IRRITATION
The irritation was reversible within 24 h after exposure in two animals and within 48 h in the third animal.
CORROSION
No corrosive effect was evident on the skin. - Other effects:
- TOXICITY SYMPTOMS / MORTALITY
No symptoms of systemic toxicity were observed in the animals during the test period and no mortality occurred.
COLOURATION
In the area of application black staining of the treated skin by the test substance was observed.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified according to CLP Regulation (EC 1272/2008)
- Conclusions:
- The test substance was found to be non irritant when applied to intact rabbit skin.
- Executive summary:
The skin irritation test was performed to rabbit skin according to the OECD guideline 404 (1981). The observed skin irritation consisted of very slight erythema (grade 1) in all animals. The irritation was reversible within 24 hours after exposure in two animals and within 48 hours in the third animal. In the area of application, black staining of the treated skin by test substance was observed. No corrosive effect occurred on the skin and no signs of systemic intoxication were observed in any of the rabbits.
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