4-chlorobenzaldehyde

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-chlorobenzaldehyde

EC Number:

203-247-4

EC Name:

4-chlorobenzaldehyde

Cas Number:

104-88-1

Molecular formula:

C7H5ClO

IUPAC Name:

4-chlorobenzaldehyde

Details on test material:

- Name of test material (as cited in study report): 4-Chlorobenzaldehyde
- Physical state: crystalline
- Analytical purity: technically pure
- Lot/batch No.: 2593

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Breeder Winkelmann, Borchen, Germany
- Weight at study initiation: 160-200 g
- Housing: Makrolon cages typ III
- Diet ( ad libitum): "Altromin R-Haltungsdiät"
- Water (ad libitum): yes


ENVIRONMENTAL CONDITIONS
- Temperature (°C): yes
- Humidity (%): yes
- Air changes (per hr): yes
- Photoperiod (hrs dark / hrs light): yes

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

peanut oil

Details on dermal exposure:

TEST SITE
- Area of exposure: shaved back skin
- coverage: wrapped


REMOVAL OF TEST SUBSTANCE
- Washing: after 24 h exposure rinsed with water and soap


TEST MATERIAL
- Amount applied: 8-10 drops in peanut oil, undiluted

Duration of exposure:

24 h

Doses:

males: 2500 or 5000 mg/kg bw
females: 1000, 2500 or 5000 mg/kg bw

No. of animals per sex per dose:

1000 or 2500 mg/kg bw : 5 animals
5000 mg/kg bw : 10 animals

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

- 2/10 females in the high dose group died after 48 hours

Gross pathology:

- after necropsy of dead animals there were findings in lungs, liver, kidneys and spleen
- animals necropsied after 14 days observation period showed no signs of pathological changes in organs examined

Any other information on results incl. tables

Dose [mg/kg bw]	Dead animals	Animals with symptoms	Animals treated
	male
2500	0	0	5
5000	0	10	10
	female
1000	0	0	5
2500	0	5	5
5000	2	10	10

Applicant's summary and conclusion

Executive summary:

In this acute dermal toxicity study groups Wistar rats were dermally exposed to p-Chlorobenzaldehyde (technical pure) in peanut oil at doses of 1000 (5 females), 2500 (5/sex) or 5000 mg/kg bw (10/sex) for 24 hours to the shaved back skin. Animals then were observed for 14 days.

Rats showed signs of toxicity until day 6 of the observation period.

Mortality occured in two out of 10 female animals of the highest dose group. After necropsy there were findings in lungs, liver, kidneys and spleen.

During terminal necropsy of surviving animals of all other groups no pathological changes were noted.

The LD 50 value was established to be > 5000 mg/kg bw for both sexes.