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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
EC Number:
303-085-5
EC Name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
Cas Number:
94158-14-2
Molecular formula:
C9H11NO3.ClH
IUPAC Name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
Test material form:
solid: crystalline

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h

Results and discussion

Effect concentrations
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
106 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
ln this study AMINOL (A098) was found to be toxic to zebrafish after 96 h at the nominal concentration level of 100 mg/L and higher. The LC50 (96 h) was 106 mg/L (81- 138 mg/L), the NOEC (96 h) was the test item concentration 50 mg/L (all nominal concentrations).
Executive summary:

The acute toxicity of the test item AMINOL (A098) {batch number: R0075037) to fish (zebrafish) was determined according to OECD-Guideline for Testing of Chemieals No. 203 (1992) from 2003-08-18 to 2003-08-22 at lhe DR.U.NOACK-LABORATORIUM FüR ANGEWANDTE BIOLOGIE, 31157 Sarstedt, Germany. A static test design was chosen with nominal concentrations of 12.5 - 25 - 50 - 100 - 200 - 400 mg/L. Duration of the test was 96 h. 7 test organisms were exposed to each test concentration and control. Water quality parameters pH-value, temperature and oxygen saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits. The control and test item concentrations of AMINOL (A098) were analytically verified via HPLC-UV at test start and end er whenever 100 % mortality occurred (for results see part 9.2). All effect values are based on the nominal concentrations of the test item. The acute toxicity of the test item AMINOL is summarized in the following table:

Table 1: LC-Values with 95 % Confidence lntervals (based on nominal concentrations)

Test duration [h]

LC50[mg/L]

P=95% [mg/L]

2

>400

Not applicable

24

>400

Not applicable

48

294

89-968

72

106

81-138

96

106

81-138

LC100

Lowest test item concentration with 100% mortality after 96 h

400 mg/L

LC0

Highest test item concentration with 0% mortality after 96 h

25 mg/L

NOEC (0-96 h)

No observerd effect concentration

*mortality of 1/7 fish is considered not to be relevant

50 mg/L*

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