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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
presumably 1974
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Only results given. Publication has lack of methodical details

Data source

Referenceopen allclose all

Reference Type:
study report
Report date:
Reference Type:
Final Report on the Safety Assessment of Acetyl Triethyl Citrate, Acetyl Tributyl Citrate, Acetyl Trihexyl Citrate, and Acetyl Trioctyl Citrate
Cosmetic Ingredient Review
Bibliographic source:
International Journal of Toxicology, 21(Suppl. 2):1–17

Materials and methods

Test guideline
no guideline followed
Principles of method if other than guideline:
1.) 3 male albino rabbits were used. The abdomen were closely clipped and 1 mL/kg bw was placed on the intact skin daily for 4 days. The animals were observed daily and for a period of 36 h after the last application.

2.) 3 male albino rabbits were used. 1 mL/kg was placed on the closely clipped abdomen for a total of 18 applications (6 d per week). Animals were observed daily and for a period of two weeks following the last application.

GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Triethyl O-acetylcitrate
EC Number:
EC Name:
Triethyl O-acetylcitrate
Cas Number:
Molecular formula:
triethyl 2-acetoxypropane-1,2,3-tricarboxylate

Test animals

other: Albino
Details on test animals or test system and environmental conditions:
no further data

Test system

Type of coverage:
not specified
Preparation of test site:
other: intact / abraded
not specified
not specified
Amount / concentration applied:
1 mL/kg bw (ca. 1000 mg/kg)
Duration of treatment / exposure:
4 - 18 applications
Observation period:
36 h to 2 weeks after last application
Number of animals:
Details on study design:
see above

Results and discussion

In vivo

Remarks on result:
no indication of irritation
Irritant / corrosive response data:
No irritant effects noted

Any other information on results incl. tables

Individual scores were not given. Examination time points: 36 h to 2 weeks after last application.

The skin irritation potential of Acetyl Triethyl Citrate was evaluated using three male albino rabbits. The test substance (1 cc/kg body weight) was inuncted onto intact abdominal skin that had been clipped free of hair. This procedure was repeated daily for 4 days. The animals were observed daily for up to 36 hours after the last application. Acetyl Triethyl Citrate did not induce skin irritation in any of the three rabbits tested .

In another study, Acetyl Triethyl Citrate (1 cc/kg body weight) was inuncted onto intact abdominal skin of each of three male albino rabbits for a total of 18 applications (6 days/week). The animals were then observed over a period of 2 weeks. Body weights were normal throughout the study. Skin irritation was not observed in any of the animals .

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
ATEC was not skin irritating. Classification criteria are not meet.
Executive summary:

In all of the 2 trials there was no indication for skin irritation.