Thiocyanic acid, (1,3,8,10-tetrahydro-1,3,8,10-tetraoxoanthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-2,9-diyl)di-3,1-phenylene ester, reaction products with sodium sulfide (Na2(Sx))

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 26 - December 12, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Version / remarks:

21 July, 1997

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Thiocyanic acid, (1,3,8,10-tetrahydro-1,3,8,10-tetraoxoanthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-2,9-diyl)di-3,1-phenylene ester, reaction products with sodium sulfide (Na2(Sx))

EC Number:

271-559-8

EC Name:

Thiocyanic acid, (1,3,8,10-tetrahydro-1,3,8,10-tetraoxoanthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-2,9-diyl)di-3,1-phenylene ester, reaction products with sodium sulfide (Na2(Sx))

Cas Number:

68585-53-5

Molecular formula:

not applicable

IUPAC Name:

Reaction product of thiocyanic acid, (1,3,8,10-tetrahydro-1,3,8,10-tetraoxoanthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-2,9-diyl)di-3,1-phenylene ester with sodium sulfide

Test material form:

solid: particulate/powder

Details on test material:

Test item: Leuco Sulfur Red 14
Appearance: light red solid
CAS No: 68585-53-5
EC No: 271-559-8

Method

Target gene:

In addition to histidine and tryptophan mutation, each strain has additional mutations which enhance its sensitivity to mutagens. The uvrB (uvrA) strains are defective in excision repair. It causes the strains to be more sensitive to the mutagenic and lethal effects of a wide variety of mutagens because they cannot repair DNA damages. rfa mutation increases the permeability of the bacterial lipopolysaccharide wall for larger molecules. The plasmid pKM101 (TA98, TA100) carries the muc+ gene which participates in the error-prone "SOS" DNA repair pathway induced by DNA damage. This plasmid also carries an ampicillin resistance transfer factor (R-factor) which is used to identify its presence in the cell. The Escherichia coli strain used in this test (WP2uvrA) is also defective in DNA excision repair.

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Metabolic activation system:

Phenobarbital (PB) and β-naphthoflavone (BNF)-induced rat liver S9 fraction

Test concentrations with justification for top dose:

Initial and Confirmatory Mutation Tests: 1600, 500, 160, 50, 16, 5 and 1.6 µg/plate

Vehicle / solvent:

- Vehicle used: Dimethyl sulfoxide (DMSO)
- Justification for choice of solvent: In the preliminary Range Finding Test dimethyl sulfoxide (DMSO) was found as appropriate vehicle for preparing the test item solutions. Based on the laboratory’s historical control database, this vehicle is compatible with the survival of the bacteria and the S9 activity.

Controls

Controls 1

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 4-Nitro-1,2-phenylenediamine

Remarks:

without S9 mix, TA 98, 4 µg/plate

Controls 2

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO, ultrapure water

True negative controls:

no

Positive controls:

yes

Positive control substance:

sodium azide

Remarks:

without S9 mix; TA 100, TA 1535; 2 µg/plate

Controls 3

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Positive control substance:

9-aminoacridine

Remarks:

without S9 mix, TA 1537, 50 µg/plate

Controls 4

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO, ultrapure water

True negative controls:

no

Positive controls:

yes

Positive control substance:

methylmethanesulfonate

Remarks:

without S9 mix, E.coli WP2 uvrA, 2 µL/plate

Controls 5

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Remarks:

with S9 mix; all tested S.typhimurium strains, 2 µg/plate; E.coli WP2 vrA, 50 µg/plate

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation); preincubation

DURATION
- Preincubation period: 20 min
- Exposure duration: 48 h

NUMBER OF REPLICATIONS:
1

DETERMINATION OF CYTOTOXICITY
- Method: revertant colony numbers, backgrounf lwn density

Origin of the Bacterial Strains:
The tester strains arrived to the test facility in a form of disc cultures. The origin of the following tester strains: Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia coli WP2 uvrA:
Supplier: Trinova Biochem GmbH; (Rathenau Str. 2; D-35394 Giessen, Germany);
Manufacturer: MOLTOX INC., (P.O. BOX 1189; BOONE, NC 28607 USA).

Storage of Tester Strains:
The strains are stored at -80 ± 10ºC in the Laboratory of TOXI-COOP ZRT. in the form of lyophilized discs and in frozen permanent copies. Frozen permanent cultures of the tester strains are prepared from fresh, overnight cultures to which DMSO is added as a cryoprotective agent

Confirmation of Phenotypes of Tester Strains:
The phenotypes of the tester strains used in the bacterial reverse mutation assays with regard to membrane permeability (rfa), UV sensitivity (uvrA and uvrB), ampicillin resistance (amp), as well as spontaneous mutation frequencies are checked regularly according to Ames et al. [1][2].
Established procedures (Standard Operating Procedures) for the preparations of each batch of frozen stock culture and raw data and reports of phenotype confirmation are stored in the Laboratory of TOXI-COOP ZRT.

Each tester strain reverts spontaneously at a frequency that is characteristic for the strain. Spontaneous reversions of the test strains to histidine or tryptophan prototrophs are measured routinely in mutagenicity experiments and expressed as the number of spontaneous revertants per plate. Historical control data are included in the test report.

Procedure for Bacterial Cultures:
The frozen bacterial cultures were thawed at room temperature and 200 µL inoculum was used to inoculate each 50 mL of Nutrient Broth No. 2 for the overnight cultures in the assay. The cultures were incubated for approximately 10-14 hours in a 37oC Benchtop Incubator Shaker.

Viability and the Cell Count of the Testing Bacterial Cultures:
The viability of each testing culture was determined by plating 0.1 mL of the 10E-5, 10E-6, 10E-7 and 10E-8 dilutions of cultures on nutrient agar plates. The viable cell number of the cultures was determined by manual colony counting.

Media
The Minimal Glucose Agar (MGA) Plates:
Ready-to-use minimal glucose agar (MGA) plates were used in the study. The origin of the ready-to use MGA plates:
Supplier: VWR International;
Manufacturer: Merck Life Science GmbH, Germany.
Certificates of Analysis1) were obtained from the supplier.
Typical composition (g/1000 mL) of MGA plates:
Glucose 20.0 g
Magnesium sulfate 0.2 g
Citric acid 2.0 g
di-Potassium hydrogenphosphate 10.0 g
Sodium ammonium hydrogenphosphate 3.5 g
Agar agar 13.0 g
1) Batch No.:138386; Expiry date: 07 September 2016; (used in the Informatory Toxicity Test)
Batch No.:139234; Expiry date: 24 October 2016; (used in the Initial and Confirmatory Mutation Tests)

Nutrient Broth No. 2
Nutrient broth No. 2. 25.0 g
Ultrapure water ad 1000.0 mL
Sterilization for 20 minutes was performed at 121˚C in an autoclave.

utrient Agar
Nutrient Agar 20.0 g
Ultrapure water ad 1000.0 mL
Sterilization for 20 minutes was performed at 121˚C in an autoclave.

Top Agar for Salmonella typhimurium Strains
Agar solution:
Agar Bacteriological 4.0 g
NaCl 5.0 g
Ultrapure water ad 1000.0 mL
Sterilization for 20 minutes was performed at 121˚C in an autoclave.

Histidine – Biotin solution (0.5 mM):
D-Biotin 122.2 mg
L-Histidine•HCl H2O 104.8 mg
Ultrapure water ad 1000.0 mL
Sterilization was performed by filtration through a 0.22 µm membrane filter.

Complete Top Agar for Salmonella typhimurium strains:
Histidine – Biotin solution (0.5 mM) 100.0 mL
Agar solution 900.0 mL

Top Agar for Escherichia coli Strain
Tryptophan solution (2 mg/mL):
L-Tryptophan 2000.0 mg
Ultrapure water ad 1000.0 mL
Sterilization was performed by filtration through a 0.22 µm membrane filter.

Complete Top Agar for Escherichia coli strain:
Nutrient Broth by 5.4.2 50.0 mL
Tryptophan solution (2 mg/mL) 2.5 mL
Agar solution by 5.4.4 947.5 mL

Metabolic Activation System

The test bacteria were also exposed to the test item in the presence of an appropriate metabolic activation system, which is a cofactor-supplemented post-mitochondrial fraction (S9).

Rat Liver S9 Fraction
The S9 fraction of Phenobarbital (PB) and β-naphthoflavone (BNF)-induced rat liver was provided by Trinova Biochem GmbH (Rathenau Str. 2.; D-35394 Giessen, Germany; Manufacturer: MOLTOX INC., P.O. BOX 1189; BOONE, NC 28607 USA). Certificate of Analysis was obtained from the supplier1). The copies of the corresponding Quality Control & Production Certificates of the S9 fractions used are attached as Appendix VII., the originals are stored in the Laboratory of TOXI-COOP ZRT.
1) Lot Number: 3453; Expiry date: April 29, 2017; Protein content: 41.8 mg/mL
(used in the Initial Mutation Test);
Lot Number: 3582; Expiry date: February 03, 2018; Protein content: 33.7 mg/mL
(used in the Informatory Toxicity Test);
Lot Number: 3577; Expiry date: January 21, 2018; Protein content: 38.4 mg/mL
(used in the Informatory Toxicity and Initial Mutation Tests).

The S9 Mix (with Rat Liver S9)
Salt solution for S9 Mix Final concentration in S9 Mix
NADP Na 7.66 g 4 mM
D-glucose-6 phosphate Na 3.53 g 5 mM
MgCl2 1.90 g 8 mM
KCl 6.15 g 33 mM
Ultrapure water ad 1000 mL
Sterilized by filtration through a 0.22 µm membrane filter.

The complete S9 Mix was freshly prepared containing components as follows:
Ice cold 0.2 M sodium phosphate-buffer, pH 7.4 500 mL
Rat liver homogenate (S9) 100 mL
Salt solution for S9 Mix 400 mL
The S9 Mix was kept in an ice bath before it was added to the culture medium.

Hamster Liver S9 Fraction
The S9 fraction of uninduced hamster liver was also provided by Trinova Biochem GmbH (Section: 5.5.1.). Certificate of Analysis was obtained from the supplier2). The copy of the corresponding Quality Control & Production Certificate of the S9 fraction used is attached as Appendix VII., the original is stored in the Laboratory of TOXI-COOP ZRT.
2) Lot Number: 3525; Expiry date: September 17, 2017; Protein content: 39.7 mg/mL
(used in the Confirmatory Mutation Test);

The S9 Mix (with Hamster Liver S9)
Salt solution for S9 Mix: Final concentration in S9 Mix:
NADP Na 15.31 g 4 mM
NADH Na2 x H2O 7.27 g 2 mM
FMN (Riboflavine-5’-phosphate-sodium salt) x H2O 4.96 g 2 mM
D-glucose-6 phosphate Na 28.20 g 20 mM
MgCl2 3.80 g 8 mM
KCl 12.31 g 33 mM
Ultrapure water ad 1000 mL

Sterilization was performed by filtration through a 0.22 µm membrane filter.

The complete S9 Mix was freshly prepared containing components as follows (per 1000 mL):
Ice cold 0.2 M sodium phosphate-buffer, pH 7.4 500 mL
Hamster liver homogenate (S9) 300 mL
Salt solution for S9 Mix 200 mL
D-glucose-6-phosphate-dehydrogenase 2800 U
Before adding to the culture medium the S9 Mix was kept in an ice bath.

Sodium Phosphate Buffer (0.2 M, pH 7.4)
Solution A:
Na2HPO4 x 12H2O 71.63 g
Ultrapure water ad 1000 mL
Solution B:
NaH2PO4 x H2O 27.6 g
Ultrapure water ad 1000 mL

Solution A 880 mL
Solution B 120 mL*
* The components were mixed in the above ratio; thereafter the pH was checked and corrected. The correction was performed with admixture of the solution A or B.
After the pH setting the sterilization was performed by filtration through a 0.22 µm membrane filter.

Rationale for test conditions:

Justification of concentrations:
Choice of the concentrations was done on the basis of a Solubility Test and a concentration Range Finding Test (Informatory Toxicity Test). Based on the solubility test, the stock solution with a concentration of 50 mg/mL was prepared in the vehicle and diluted in at least 6 steps by factor of approximately √10. The revertant colony numbers and the inhibition of the background lawn of auxotrophic cells of two of the tester strains (Salmonella typhimurium TA98, TA100) were determined at the concentrations of 5000, 1600, 500, 160, 50, 16 and 5 µg/plate of the test item.
The revertant colony numbers of vehicle control plates in both strains with and without S9 Mix were in line with the corresponding historical control data ranges. The positive control treatments showed the expected, biological relevant increases in induced revertant colonies in both tester strains.
Precipitate was noticed on the plates at the concentrations of 5000 and 1600 μg/plate in absence and presence of metabolic activation (±S9 Mix).
While the precipitate disturbed the scoring at 5000 μg/plate (±S9 Mix), it was not interfering at 1600 μg/plate (±S9 Mix).
For confirmation of manual evaluations (made by naked eye) all of the plates were checked for colony and background lawn development by microscope at 40X magnification. At this magnification test item particles were noticed down to and including the concentration of 16 μg/plate (± S9 Mix).

Evaluation criteria:

The colony numbers on the controls (untreated, vehicle, positive) and the test plates were determined (counted manually), the mean values and appropriate standard deviations and mutation rates were calculated.
A test item is considered mutagenic if:
- a dose–related increase in the number of revertants occurs and/or;
- a reproducible biologically relevant positive response for at least one of the dose groups occurs in at least one strain with or without metabolic activation.

An increase is considered biologically relevant if:
- in strain Salmonella typhimurium TA100 the number of reversions is at least twice as high as the reversion rate of the vehicle control,
- in strain Salmonella typhimurium TA98, TA1535, TA1537 and Escherichia coli WP2 uvrA the number of reversions is at least three times higher than the reversion rate of the vehicle control.

According to the guidelines, the biological relevance of the results is the criterion for the interpretation of results, a statistical evaluation of the results is not regarded as necessary.

Criteria for a Negative Response:
A test item is considered non-mutagenic if it produces neither a dose-related increase in the number of revertants nor a reproducible biologically relevant positive response at any of the dose groups, with or without metabolic activation.

Statistics:

The mean values and appropriate standard deviations and mutation rates were calculated by EXCEL software

Results and discussion

Test results

Test results 1

Key result

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Key result

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 3

Key result

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 4

Key result

Species / strain:

S. typhimurium TA 1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 5

Key result

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: above 1600 µg/plate, determined in an informatory toxicity test

Any other information on results incl. tables

Table 4: Results of the Initial Mutation Test

Initial Mutation Test (Plate Incorporation Test)
Concentrations (µg/plate)	Salmonella typhimuriumtester strains																Escherichiacoli
	TA 98				TA 100				TA 1535				TA 1537				WP2uvrA
	-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9
Mean values of revertants per plate Mutation rate (MR)	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR
Untreated Control	21.7	1.33	26.0	0.88	86.0	1.01	117.3	1.27	9.3	0.88	11.0	0.94	7.3	1.29	4.7	0.64	18.3	0.83	19.3	0.94
DMSO Control	16.3	1.00	29.7	1.00	85.0	1.00	92.3	1.00	10.7	1.00	11.7	1.00	5.7	1.00	7.3	1.00	22.0	1.00	20.7	1.00
Ultrapure Water Control	–	–	–	–	102.7	1.00	–	–	12.7	1.00	–	–	–	–	–	–	19.7	1.00	–	–
1600	19.0	1.16	19.3	0.65	104.3	1.23	121.3	1.31	9.7	0.91	13.0	1.11	10.7	1.88	6.0	0.82	15.3	0.70	21.7	1.05
500	34.0	2.08	21.0	0.71	108.0	1.27	131.7	1.43	13.3	1.25	9.7	0.83	11.7	2.06	9.0	1.23	14.7	0.67	22.0	1.06
160	23.0	1.41	22.3	0.75	105.3	1.24	139.3	1.51	11.0	1.03	10.3	0.89	11.7	2.06	6.3	0.86	23.7	1.08	19.3	0.94
50	23.0	1.41	22.7	0.76	102.7	1.21	122.0	1.32	11.3	1.06	16.0	1.37	8.7	1.53	4.3	0.59	24.0	1.09	18.3	0.89
16	24.7	1.51	23.0	0.78	102.0	1.20	136.3	1.48	13.7	1.28	11.3	0.97	12.0	2.12	7.7	1.05	17.7	0.80	21.0	1.02
5	22.0	1.35	19.0	0.64	96.0	1.13	123.0	1.33	8.0	0.75	10.3	0.89	10.7	1.88	8.0	1.09	14.3	0.65	27.7	1.34
1.6	22.3	1.37	22.3	0.75	93.0	1.09	110.3	1.19	9.3	0.88	12.0	1.03	6.0	1.06	7.7	1.05	13.7	0.62	18.0	0.87
NPD (4 µg)	274.7	16.82	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
SAZ (2 µg)	–	–	–	–	1240.0	12.08	–	–	561.7	44.34	–	–	–	–	–	–	–	–	–	–
9AA (50 µg)	–	–	–	–	–	–	–	–	–	–	–	–	597.3	105.41	–	–	–	–	–	–
MMS (2 µL)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	892.0	45.36	–	–
2AA (2 µg)	–	–	2336.0	78.74	–	–	2317.3	25.10	–	–	183.7	15.74	–	–	169.7	23.14	–	–	–	–
2AA (50 µg)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	220.0	10.65

MR: Mutation Rate;          NPD: 4-Nitro-1,2-phenylenediamine; SAZ: Sodium azide; 9AA: 9-Aminoacridine; MMS: Methyl methanesulfonate; 2AA: 2-aminoanthracene

Remarks:           DMSO was applied as vehicle of the test item and positive control substances: NPD, 9AA and 2AA and the ultrapure water was applied as vehicle for the SAZ and MMS. The mutation rate of the test item and the untreated control is given referring to the DMSO. The mutation rate of the NPD, 9AA and 2AA is given referring to the DMSO and the mutation rate of the SAZ and MMS positive control is given referring to the ultrapure water.

Table 5: Results of the Confirmatory Mutation Test

Confirmatory Mutation Test (Pre-Incubation Test)
Concentrations (µg/plate)	Salmonella typhimuriumtester strains																Escherichia coli
	TA 98				TA 100				TA 1535				TA 1537				WP2uvrA
	-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9		-S9		+S9
Mean values of revertants per plate Mutation rate (MR)	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR	Mean	MR
Untreated Control	18.0	0.95	17.7	0.98	97.3	1.20	109.7	1.02	10.7	1.23	12.0	0.97	13.7	1.00	5.7	0.81	18.0	0.93	30.0	1.22
DMSO Control	19.0	1.00	18.0	1.00	81.3	1.00	108.0	1.00	8.7	1.00	12.3	1.00	13.7	1.00	7.0	1.00	19.3	1.00	24.7	1.00
Ultrapure Water Control	–	–	–	–	90.3	1.00	–	–	14.3	1.00	–	–	–	–	–	–	22.3	1.00	–	–
1600	29.0	1.53	16.7	0.93	106.0	1.30	102.0	0.94	9.7	1.12	10.7	0.86	12.0	0.88	10.3	1.48	17.3	0.90	34.7	1.41
500	23.3	1.23	13.0	0.72	88.7	1.09	101.7	0.94	6.3	0.73	9.3	0.76	13.0	0.95	10.3	1.48	21.0	1.09	26.3	1.07
160	20.7	1.09	18.3	1.02	82.7	1.02	106.0	0.98	8.0	0.92	11.3	0.92	12.7	0.93	7.0	1.00	18.3	0.95	26.7	1.08
50	20.3	1.07	17.3	0.96	87.3	1.07	104.7	0.97	8.0	0.92	10.0	0.81	9.7	0.71	7.3	1.05	21.0	1.09	20.3	0.82
16	19.7	1.04	17.0	0.94	92.3	1.14	90.0	0.83	8.7	1.00	11.3	0.92	12.0	0.88	7.0	1.00	20.3	1.05	29.0	1.18
5	21.7	1.14	13.3	0.74	92.7	1.14	109.3	1.01	7.3	0.85	13.0	1.05	11.3	0.83	5.7	0.81	25.0	1.29	22.7	0.92
1.6	22.7	1.19	17.3	0.96	90.3	1.11	107.3	0.99	7.7	0.88	10.0	0.81	10.7	0.78	9.3	1.33	20.3	1.05	19.0	0.77
NPD (4 µg)	188.7	9.93	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
SAZ (2 µg)	–	–	–	–	1248.0	13.82	–	–	794.7	55.44	–	–	–	–	–	–	–	–	–	–
9AA (50 µg)	–	–	–	–	–	–	–	–	–	–	–	–	264.0	19.32	–	–	–	–	–	–
MMS (2 µL)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	824.0	36.90	–	–
2AA (2 µg)	–	–	684.0	38.00	–	–	984.0	9.11	–	–	160.0	12.97	–	–	93.3	13.33	–	–	–	–
2AA (50 µg)	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	146.7	5.95

MR: Mutation Rate;          NPD: 4-Nitro-1,2-phenylenediamine; SAZ: Sodium azide; 9AA: 9-Aminoacridine; MMS: Methyl methanesulfonate; 2AA: 2-aminoanthracene

Remarks:           DMSO was applied as vehicle of the test item and positive control substances: NPD, 9AA and 2AA and the ultrapure water was applied as vehicle for the SAZ and MMS. The mutation rate of the test item and the untreated control is given referring to the DMSO. The mutation rate of the NPD, 9AA and 2AA is given referring to the DMSO and the mutation rate of the SAZ and MMS positive control is given referring to the ultrapure water.

 

Applicant's summary and conclusion

Conclusions:

In an in vitro bacterial reverse mutation assay (Ames) according to OECD guideline 471, the test item did not show mutagenic properties.

Executive summary:

The test item was tested with regard to a potential mutagenic activity using the Bacterial Reverse Mutation Assay (Ames) according to OECD guideline 471.

The experiments were carried out using histidine-requiring auxotroph strains of Salmonella typhimurium (Salmonella typhimuriumTA98, TA100, TA1535 and TA1537), and the tryptophan-requiring auxotroph strain of Escherichia coli (Escherichia coliWP2uvrA) in the presence and absence of a post mitochondrial supernatant (S9) prepared from livers of Phenobarbital/b-naphthoflavone-induced rats. The study included a Preliminary Solubility Test, a Preliminary Concentration Range Finding Test (Informatory Toxicity Test), an Initial Mutation Test (Plate Incorporation Test), and a Confirmatory Mutation Test (Pre-Incubation Test).

Based on the results of the Solubility Test and the Concentration Range Finding Test the test item was dissolved in dimethyl sulfoxide (DMSO). Based on the results of the preliminary Concentration Range Finding Test (Informatory Toxicity Test) the following concentrations of the test item were prepared and investigated in the Initial and Confirmatory Mutation Tests: 1600; 500; 160; 50; 16; 5 and 1.6 µg/plate. The selection of the concentration range was based on the recommendations in OECD 471 guideline. At the concentration choice the guideline criterion for non-toxic test compounds with limited solubility was taken into consideration and accordingly the test item was investigated up to and including the precipitated concentration level (observation made by naked eye).

In the main experiments (when evaluated by naked eye) test item eye precipitate was noticed after about 48 hours incubation on the plates in the examined strains at the concentration of 1600 µg/plate in absence and in the presence of S9 following the plate incorporation and pre-incubation procedures. The obtained precipitate did not interfere with the scoring of the colonies in any case. The test item did not show inhibitory, cytotoxic effects in the performed experiments. The colony and background lawn development was not affected in any case; the obtained revertant colony number decreases (compared to the revertant colony numbers of the vehicle control) remained within the biological variability range of the applied test system. The revertant colony numbers of vehicle control (dimethyl sulfoxide (DMSO) plates with and without S9 Mix demonstrated the characteristic mean number of spontaneous revertants that was in line with the corresponding historical control data ranges. The reference mutagen treatments (positive controls) showed the expected, biological relevant increases (more than 3-fold increase)in induced revertant colonies and the number of revertants fell in the corresponding historical control ranges, thereby meeting the criteria for the positive control in all experimental phases, in all tester strains.

No biologically relevant increases were observed in revertant colony numbers of any of the five test strains following treatment with the test item at any concentration level, either in the presence or absence of metabolic activation (S9 Mix) in the performed experiments.

The reported data of this mutagenicity assay show that under the experimental conditions applied, the test item did not induce gene mutations by base pair changes or frameshifts in the genome of the strains used.