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EC number: 230-237-7 | CAS number: 6976-37-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No. of test material: Product no. B4429, Lot no. SLBK5235V
- Expiration date of the lot/batch: 30 April 2018
- Purity test date: na
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: no - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L
- Sampling method: - no data
- Sample storage conditions before analysis: not applicable - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- no difficulties encountered (0.5 mg test substance/mL; dissolved in ultra-pure water) - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus
- Justification for species other than prescribed by test guideline: -
- Source: Laboratory of Hydrobiology, 2100 Gödöllö, Kotlan S. u. 3, Hungary
- Age of parental stock (mean and range, SD): not available
- Feeding during test: no
- Food type: not applicable
- Amount: not applicable
- Frequency: not applicable
ACCLIMATION
- Acclimation period: at least 48h
- Acclimation conditions (same as test or not): similar or same
- Type and amount of food: concentrated algal suspension
- Feeding frequency: not available
- Health during acclimation (any mortality observed): not available
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- none
- Post exposure observation period:
- no
- Hardness:
- 249 mg/L (as CaCO3)
- Test temperature:
- 20.1 - 20.2 °C
- pH:
- 7.90- 8.07
- Dissolved oxygen:
- 7.96 - 8.07 mg/L
- Salinity:
- no information available
- Conductivity:
- no information available
- Nominal and measured concentrations:
- The study was conducted as a limit test with the concentration of 100 mg/L. The concentrations reported as nominal as recovery was 99% (start) and 102% (end).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: fill volume: 40 mL
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): not applicable
- Renewal rate of test solution (frequency/flow rate): not applicable
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: not available
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (ISO medium acc. to OECD TG 202)
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16/8 (light/dark)
- Light intensity: not available
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation (24 and 48h)
VEHICLE CONTROL PERFORMED: not applicable
RANGE-FINDING STUDY
- Test concentrations: 0.01 - 100 mg/L
- Results used to determine the conditions for the definitive study: no immobilisation observed at any concentration - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (non-concurrent, but tested on 20-21.09.2016)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: none
- Observations on body length and weight: none
- Other biological observations: none reported
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid?
- Relevant effect levels: 24h EC50:1.57 mg/L (performed on 20.-21.09.16)
- Limit test: no
- Dose-response test: yes - Validity criteria fulfilled:
- yes
- Executive summary:
The acute toxicity of the substance was investigated in a GLP-study according to OECD test guideline 202 using Daphnia magna.
The study was performed using one concentration containing 100 mg/L with a treatment period of 48 hours under static conditions. The immobilisation of Daphnia was observed at 24h and 48h.
At the start and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV. The recovery after 48 hours was 102 % of the start concentration. Therefore, the determination of the results was based on the nominal concentrations.
The EC50 of the positive reference control potassium dichromate were tested in a non-current reference test. The values lay within the normal range of the laboratory.
As the substance caused no toxicity, a NOEC of 100 mg/L and an EC50 of >100 mg/L were determined.
Reference
In a GLP-study according to OECD test guideline 202 the substance did show no toxicity to daphnia magna at the limit test concentration of 100 mg/L under static conditions. Therefore, it has a NOEC of 100 mg/L and an EC50 of >100 mg/L.
Description of key information
In a GLP-study according to OECD test guideline 202 (static) the substance did show no toxicity to daphnia magna at the limit test concentration of 100 mg/L. Therefore, it has a NOEC of 100 mg/L and an EC50 of >100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
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